IFN- gamma R alpha Antibody (2E2) recommended for detection of IFN-γRα of mouse, rat and human origin by WB, IP, IF and FCM

IFN-γRα Antibody (2E2): sc-12753

IFN-γRα Antibody (2E2) is rated 4.8 out of 5 by 4.
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Datasheets

    • IFN-γRα Antibody (2E2) is a Armenian hamster monoclonal IgG provided at 200 µg/ml
    • epitope mapping the extracellular domain of IFN-γRα
    • Anti-IFN- gamma R alpha Antibody (2E2) is recommended for detection of IFN-γRα of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-IFN- gamma R alpha Antibody (2E2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-γRα (2E2): sc-12753.

IFN-γRα Antibody (2E2) HRP sc-12753 HRP Is this antibody applicable for western blot? Is the secondary antibody necessary for ECL detection?

Asked by: jwu51
Thank you for your question. Yes, this antibody is applicable for Western blot (WB). If you purchase the HRP conjugated primary antibody (sc-12753 HRP), then no secondary antibody is needed for ECL detection.
Answered by: Technical Support
Date published: 2019-04-13

Can IFN-γRα (2E2): sc-12753 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IFN-γRα (2E2): sc-12753 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great publishable staining in paraffin Great publishable staining in paraffin-embedded human hair follicles. -SCBT Publication Review
Date published: 2015-06-02
Rated 5 out of 5 by from Published good quality flow cytometry data Published good quality flow cytometry data in human eosinophil granules. -SCBT Publication Review
Date published: 2015-05-14
Rated 4 out of 5 by from Produced robust FCM data for mouse peripheral Produced robust FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Published good FCM data using moouse Th1 and Th2 Published good FCM data using moouse Th1 and Th2. -SCBT Publication Review
Date published: 2015-05-05
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