Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Iba1 CRISPR/Cas9 KO Plasmid (m): sc-419058
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Iba1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Iba1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Iba1 Antibody (1022-5): sc-32725
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Iba1 CRISPR/Cas9 KO Plasmid (m) | sc-419058 | 20 µg | $397.00 |
Overview
Aif1 encodes ionized calcium-binding adapter molecule 1 (Iba1), a cytosolic EF-hand protein highly enriched in microglia and other myeloid-lineage cells. Iba1 participates in actin cytoskeleton remodeling, membrane ruffling, and phagocytosis, supporting innate immune surveillance and inflammatory signaling programs in the central nervous system. Through interactions that influence Rac/Rho-dependent motility and responses to calcium dynamics, Iba1 contributes to microglial activation states and tissue injury responses. Altered Aif1/Iba1 expression is widely used as a marker of neuroinflammation and has been associated with pathological microglial phenotypes in neurodegenerative and neuroimmune disease models.
Iba1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aif1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aif1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aif1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Iba1 protein expression.
This CRISPR knockout system enables efficient generation of Aif1-deficient cell models for investigation of Iba1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aif1 exon(s) critical for Iba1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aif1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Iba1 CRISPR/Cas9 KO Plasmid (m) and Iba1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aif1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Iba1 HDR Plasmid (m) and Iba1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aif1 homology arms to support homology-directed repair at defined Aif1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.