HXK III Antibody (A-9): sc-74488

HXK III Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects HXK III of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HXK III antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HXK III plays a crucial role in glucose metabolism, catalyzing the conversion of glucose to glucose-6-phosphate, which is the first step in the glycolytic pathway. This enzymatic activity is vital for cellular energy production and metabolic regulation, particularly in tissues with high energy demands. Among the four hexokinase isoenzymes, HXK III exhibits the highest affinity for glucose, making HXK III essential for maintaining glucose homeostasis in cells. HXK III is particularly significant in tissues that require rapid glucose utilization, such as the brain and muscle, where HXK III helps to ensure a steady supply of energy. Understanding HXK III function and regulatory mechanisms is important for elucidating metabolic disorders and developing therapeutic strategies for conditions such as diabetes.

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HXK III Antibody (A-9) References:

1. The overexpressed hexahistidine-tagged human hexokinase type III is inhibited by D-glucose.  |  Palma, F., et al. 2002. Prep Biochem Biotechnol. 32: 393-403. PMID: 12455831
2. Human glucokinase gene: isolation, characterization, and identification of two missense mutations linked to early-onset non-insulin-dependent (type 2) diabetes mellitus.  |  Stoffel, M., et al. 1992. Proc Natl Acad Sci U S A. 89: 7698-702. PMID: 1502186
3. Glucose phosphorylation in tumor cells. Cloning, sequencing, and overexpression in active form of a full-length cDNA encoding a mitochondrial bindable form of hexokinase.  |  Arora, KK., et al. 1990. J Biol Chem. 265: 6481-8. PMID: 2318862
4. Hexokinase 3 dysfunction promotes tumorigenesis and immune escape by upregulating monocyte/macrophage infiltration into the clear cell renal cell carcinoma microenvironment.  |  Xu, W., et al. 2021. Int J Biol Sci. 17: 2205-2222. PMID: 34239350
5. Hexokinase 3 enhances myeloid cell survival via non-glycolytic functions.  |  Seiler, K., et al. 2022. Cell Death Dis. 13: 448. PMID: 35538058
6. Chronic social stress disrupts the intracellular redistribution of brain hexokinase 3 induced by shifts in peripheral glucose levels.  |  van der Kooij, MA., et al. 2022. J Mol Med (Berl). 100: 1441-1453. PMID: 35943566
7. High iodine promotes autoimmune thyroid disease by activating hexokinase 3 and inducing polarization of macrophages towards M1.  |  Cai, T., et al. 2022. Front Immunol. 13: 1009932. PMID: 36325332
8. CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure.  |  Li, T., et al. 2023. Mol Cancer. 22: 77. PMID: 37106446
9. Reliable hexokinase 3 protein detection in human cell lines and primary tissue.  |  Mady, YH., et al. 2025. Eur J Histochem. 69: PMID: 40071468
10. Multiple forms of hexokinase in the rat: tissue distribution, age dependency, and properties.  |  Katzen, HM. and Schimke, RT. 1965. Proc Natl Acad Sci U S A. 54: 1218-25. PMID: 5219826
11. Human hexokinase II: sequence and homology to other hexokinases.  |  Deeb, SS., et al. 1993. Biochem Biophys Res Commun. 197: 68-74. PMID: 8250948
12. Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein.  |  Palma, F., et al. 1996. Mol Cell Biochem. 155: 23-9. PMID: 8717435

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HXK III Antibody (A-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HXK III Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HXK III Antibody (A-9) Specifications

Alternate Product Name: Hexokinase III

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-130

Epitope Target: HXK III

Epitope Species: human

Product Reactivity/Detection: HXK III

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HXK III Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin