Date published: 2025-11-26

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human heart extract: sc-363763

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Datasheets
  • 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
  • Western blotting positive control
  • recommended use is 50 µg (20 µl) per lane
  • extracts should be stored at -20°C and repeated freezing and thawing should be minimized
  • sample vial should be placed at 95° C for up to 5 minutes, once prior to use
  • Not available in Germany.

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Human heart extract is derived from the cardiac tissue of the human heart, an organ renowned for its role in pumping blood throughout the body. This extract is composed of a complex mixture of heart muscle cells (cardiomyocytes), connective tissue, and a rich array of proteins and enzymes that are crucial for cardiac function and metabolism. The extraction process involves the meticulous dissection of heart tissue, followed by homogenization to break down the cells and release intracellular components, and subsequent purification to isolate specific biochemical substances. In research, human heart extract provides a vital resource for studying the biochemical and physiological mechanisms underlying heart function, including muscle contraction, electrical conduction, and the regulation of heart rate and blood pressure. Scientists utilize this extract to explore the molecular basis of heart muscle metabolism and energy production, investigating how heart cells utilize nutrients and manage oxidative stress. Furthermore, it is used in studies focused on understanding the processes of cardiac hypertrophy and the molecular signaling pathways involved in heart development and adaptation. Through these applications, human heart extract contributes significantly to our knowledge of cardiovascular biology, offering insights that help explain the complex dynamics of heart health and disease.

human heart extract References:

  1. Ca2+ -dependent interaction of S100A1 with the sarcoplasmic reticulum Ca2+ -ATPase2a and phospholamban in the human heart.  |  Kiewitz, R., et al. 2003. Biochem Biophys Res Commun. 306: 550-7. PMID: 12804600
  2. Sildenafil citrate does not affect cardiac contractility in human or dog heart.  |  Corbin, J., et al. 2003. Curr Med Res Opin. 19: 747-52. PMID: 14687446
  3. The Ku protein complex interacts with YY1, is up-regulated in human heart failure, and represses alpha myosin heavy-chain gene expression.  |  Sucharov, CC., et al. 2004. Mol Cell Biol. 24: 8705-15. PMID: 15367688
  4. Circulating heart-reactive antibodies in patients with myocarditis or cardiomyopathy.  |  Neumann, DA., et al. 1990. J Am Coll Cardiol. 16: 839-46. PMID: 2229805
  5. Human IgG and Streptococcus mutans SR protein contain cross-reactive epitopes.  |  Wachsmann, D., et al. 1989. J Immunol. 143: 4257-62. PMID: 2480390
  6. Myosin: a link between streptococci and heart.  |  Krisher, K. and Cunningham, MW. 1985. Science. 227: 413-5. PMID: 2578225
  7. The cardiac autoimmune system. VII. Heart specific auto-antibodies produced in rabbits immunized with human heart.  |  Lin, TM., et al. 1972. Immunol Commun. 1: 407-20. PMID: 4217767
  8. Oxamate as a differential inhibitor of lactate dehydrogenase isoenzymes.  |  Wilkinson, JH. and Walter, SJ. 1972. Enzyme. 13: 170-6. PMID: 4650384
  9. Choline and carnitine acetyltransferases of heart.  |  White, HL. and Wu, JC. 1973. Biochemistry. 12: 841-6. PMID: 4686801
  10. Postsynthetic variants of creatine kinase MM.  |  Yasmineh, WG., et al. 1981. J Lab Clin Med. 98: 109-118. PMID: 7252320
  11. Succinyl-CoA:3-ketoacid coenzyme A transferase (SCOT): development of an antibody to human SCOT and diagnostic use in hereditary SCOT deficiency.  |  Song, XQ., et al. 1997. Biochim Biophys Acta. 1360: 151-6. PMID: 9128180
  12. Influence of some factors on immunoassays of human myoglobin.  |  Kochanowska, IE., et al. 1998. Arch Immunol Ther Exp (Warsz). 46: 85-91. PMID: 9613705
  13. Antibodies to cardiac tissue in acute ischemic heart disease☆  |  WI Heine, H Friedman, MS Mandell… - The American Journal of …, 1966 - Elsevier. June 1966,. The American Journal of Cardiology. Volume 17, Issue 6,: Pages 798-803.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

human heart extract

sc-363763
500 µg/200 µl
$118.00