HSP 90α Double Nickase Plasmid (h): sc-400088-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 90α Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSP 90α Double Nickase Plasmid (h) and HSP 90α Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSP90AA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 90α Antibody (F-2): sc-515081
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 90α Double Nickase Plasmid (h) | sc-400088-NIC | 20 µg | $410.00 | |||
HSP 90α Double Nickase Plasmid (h2) | sc-400088-NIC-2 | 20 µg | $410.00 |
HSP90AA1 encodes the molecular chaperone HSP90α, a highly abundant ATP-dependent regulator of proteostasis that supports folding, maturation, and stabilization of diverse client proteins, including kinases, nuclear receptors, and transcription factors. HSP90α operates with co-chaperones in the heat shock response and integrates into pathways governing cell-cycle progression, signal transduction, stress adaptation, and protein quality control. By buffering proteome instability, HSP90α influences the dynamics of multiprotein complexes and the turnover of signaling nodes. Dysregulation of HSP90AA1/HSP90α activity has been associated with altered stress tolerance and remodeling of oncogenic and inflammatory signaling networks, making it a frequent focus in studies of proteostasis and pathway dependency.
HSP 90α Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSP90AA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSP90AA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSP90AA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSP90AA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.