Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HS6ST1 CRISPR/Cas9 KO Plasmid (h): sc-405111
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HS6ST1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HS6ST1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HS6ST1 Antibody (C-9): sc-398231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HS6ST1 CRISPR/Cas9 KO Plasmid (h) | sc-405111 | 20 µg | $397.00 |
Overview
HS6ST1 encodes heparan sulfate 6-O-sulfotransferase 1, a Golgi-resident enzyme that catalyzes 6-O-sulfation of glucosamine residues during heparan sulfate proteoglycan maturation. This modification shapes heparan sulfate binding sites that control the distribution and signaling potency of multiple morphogens and growth factors, influencing processes such as receptor tyrosine kinase signaling, cell–matrix interactions, and developmental patterning. By tuning heparan sulfate fine structure, HS6ST1 can modulate pathways including FGF, WNT, BMP, and Hedgehog signaling in a context-dependent manner. Altered heparan sulfate sulfation programs involving HS6ST1 have been associated with dysregulated cellular communication in cancer biology and neurodevelopmental phenotypes, making it a useful node for mechanistic studies of extracellular signaling regulation.
HS6ST1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HS6ST1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HS6ST1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HS6ST1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HS6ST1 protein expression.
This CRISPR knockout system enables efficient generation of HS6ST1-deficient cell models for investigation of HS6ST1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HS6ST1 exon(s) critical for HS6ST1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HS6ST1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HS6ST1 CRISPR/Cas9 KO Plasmid (h) and HS6ST1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HS6ST1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HS6ST1 HDR Plasmid (h) and HS6ST1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HS6ST1 homology arms to support homology-directed repair at defined HS6ST1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.