HoxC8 Double Nickase Plasmid (h): sc-405407-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxC8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxC8 Double Nickase Plasmid (h) and HoxC8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXC8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxC8 Antibody (1H2): sc-517007
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxC8 Double Nickase Plasmid (h) | sc-405407-NIC | 20 µg | $410.00 | |||
HoxC8 Double Nickase Plasmid (h2) | sc-405407-NIC-2 | 20 µg | $410.00 |
HOXC8 encodes the homeobox transcription factor HoxC8, a sequence-specific DNA-binding regulator that helps pattern the anterior–posterior axis during embryogenesis and guides differentiation programs in mesodermal and neural crest–derived tissues. HoxC8 modulates gene networks controlling cell fate decisions, morphogenesis, and tissue-specific transcriptional programs through HOX-cofactor interactions and chromatin-dependent regulation. Dysregulated HOXC8 expression has been reported across multiple tumor types and is associated with altered proliferative, migratory, and epithelial–mesenchymal transition–linked transcriptional states, making it a useful node for studying developmental reprogramming and oncogenic signaling. In addition, HOXC8 activity intersects with pathways governing extracellular matrix remodeling and lineage specification, supporting mechanistic studies in development, cancer biology, and transcriptional regulation.
HoxC8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXC8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXC8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXC8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXC8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.