Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxA11 CRISPR/Cas9 KO Plasmid (h): sc-405264
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxA11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA11 Antibody (B-11): sc-393440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA11 CRISPR/Cas9 KO Plasmid (h) | sc-405264 | 20 µg | $397.00 |
Overview
HOXA11 encodes the homeobox transcription factor HoxA11, a sequence-specific DNA-binding regulator that controls developmental patterning and mesenchymal differentiation programs. In human cells, HoxA11 contributes to transcriptional networks governing limb and urogenital tract morphogenesis and is implicated in endometrial stromal decidualization through context-dependent crosstalk with steroid hormone signaling and chromatin remodeling. Dysregulated HOXA11 expression or altered regulatory landscapes have been associated with reproductive disorders and developmental anomalies, and HOX cluster perturbations are recurrent features across multiple cancer types where they can influence proliferation and lineage identity. As a nuclear transcriptional regulator, HoxA11 provides a tractable entry point for dissecting gene regulatory circuits and epigenetic state transitions in development and disease-relevant models.
HoxA11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HOXA11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HOXA11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HOXA11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxA11 protein expression.
This CRISPR knockout system enables efficient generation of HOXA11-deficient cell models for investigation of HoxA11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HOXA11 exon(s) critical for HoxA11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HOXA11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxA11 CRISPR/Cas9 KO Plasmid (h) and HoxA11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HOXA11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxA11 HDR Plasmid (h) and HoxA11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HOXA11 homology arms to support homology-directed repair at defined HOXA11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.