hnRNP C1/C2 Double Nickase Plasmid (h): sc-400993-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP C1/C2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP C1/C2 Double Nickase Plasmid (h) and hnRNP C1/C2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNRNPC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP C1/C2 Antibody (4F4): sc-32308
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP C1/C2 Double Nickase Plasmid (h) | sc-400993-NIC | 20 µg | $410.00 | |||
hnRNP C1/C2 Double Nickase Plasmid (h2) | sc-400993-NIC-2 | 20 µg | $410.00 |
HNRNPC encodes the heterogeneous nuclear ribonucleoprotein C1/C2 (hnRNP C1/C2), abundant nuclear RNA-binding proteins that assemble on nascent pre-mRNA to regulate splice site choice, mRNA maturation, and transcript stability. hnRNP C1/C2 helps enforce fidelity in RNA processing by competing with U2AF2 at cryptic polyuridine tracts, thereby limiting aberrant exonization of Alu-derived sequences and shaping isoform outputs. Through these activities it influences broader gene-expression programs linked to cell-cycle control, DNA damage responses, and proteostasis, and is frequently studied in the context of RNA dysregulation observed in cancer and neurodegenerative disease models. Altered HNRNPC expression or function has also been associated with changes in innate immune signaling and stress-adaptive transcriptional networks driven by disrupted RNA metabolism.
hnRNP C1/C2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNRNPC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNRNPC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNRNPC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNRNPC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.