HLX1 CRISPR/Cas9 KO Plasmid (h): sc-403700
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HLX1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HLX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HLX1 Antibody (1B9): sc-293328
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLX1 CRISPR/Cas9 KO Plasmid (h) | sc-403700 | 20 µg | $397.00 |
Overview
HLX (H2.0-like homeobox) encodes the homeodomain transcription factor HLX1, a regulator of developmental gene expression programs and lineage-specific differentiation. HLX1 influences hematopoietic and immune cell maturation by coordinating transcriptional networks that govern proliferation, survival, and cell fate decisions, linking it to pathways controlling cytokine-responsive signaling and downstream transcriptional regulation. Dysregulated HLX expression has been reported in contexts relevant to hematologic and immune-associated disease biology, making it a useful target for studying mechanisms of aberrant differentiation and transcriptional reprogramming. As a nuclear DNA-binding protein, HLX1 is also applied in research on enhancer-promoter control, chromatin-dependent regulation, and developmental transcription factor circuitry.
HLX1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HLX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HLX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HLX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HLX1 protein expression.
This CRISPR knockout system enables efficient generation of HLX-deficient cell models for investigation of HLX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HLX exon(s) critical for HLX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HLX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HLX1 CRISPR/Cas9 KO Plasmid (h) and HLX1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HLX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HLX1 HDR Plasmid (h) and HLX1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HLX homology arms to support homology-directed repair at defined HLX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.