Histone cluster 1 H2BK Double Nickase Plasmid (h): sc-417678-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone cluster 1 H2BK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone cluster 1 H2BK Double Nickase Plasmid (h) and Histone cluster 1 H2BK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HIST1H2BK. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone cluster 1 H2BK Double Nickase Plasmid (h) | sc-417678-NIC | 20 µg | $410.00 | |||
Histone cluster 1 H2BK Double Nickase Plasmid (h2) | sc-417678-NIC-2 | 20 µg | $410.00 |
HIST1H2BK encodes a replication-dependent H2B family histone that integrates into nucleosomes to compact genomic DNA and regulate chromatin accessibility. Through its role in nucleosome assembly, histone exchange, and higher-order chromatin organization, Histone cluster 1 H2BK influences transcriptional control, DNA replication timing, and DNA damage signaling and repair. Post-translational modifications on H2B, including ubiquitination and phosphorylation, participate in crosstalk with other histone marks that coordinate cell-cycle progression and genome stability. Dysregulated histone gene dosage or altered chromatin states are broadly linked to oncogenic transcriptional programs and aberrant DNA repair, making HIST1H2BK a useful locus for studying epigenetic mechanisms relevant to disease-associated chromatin remodeling.
Histone cluster 1 H2BK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HIST1H2BK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HIST1H2BK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HIST1H2BK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HIST1H2BK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.