Date published: 2025-11-4

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His-Tag Antibody (His17): sc-51946

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Datasheets
  • His-Tag Antibody (His17) is a mouse monoclonal IgG1, cited in 8 publications, provided at 100 µg/ml
  • raised against a His-tagged recombinant protein
  • recommended for detection of fusion proteins encoded by polyhistidine expression vectors by WB
  • See His-Tag (H-3): sc-8036 for His-Tag antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for His-Tag Antibody (His17). This work is in progress.

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His-Tag Antibody (His17) is a mouse monoclonal IgG1 antibody raised against a His-tagged recombinant protein, specifically designed for the detection of His-tagged fusion proteins in applications such as western blotting (WB). The His-tag, typically consisting of a sequence of six histidine residues, is widely used in molecular biology to facilitate the purification and detection of recombinant proteins. His-tagged proteins bind strongly to metal ions like nickel or cobalt, allowing for efficient purification through immobilized metal affinity chromatography. Understanding the cellular location of His-tagged proteins is crucial, as this knowledge provides insights into function, interaction with other cellular components, and involvement in various biological processes. Detecting His-tagged protein localization can reveal important information about protein trafficking, signaling pathways, and cellular dynamics. Anti-His-Tag antibody (His17) enables researchers to accurately identify and study His-tagged proteins within cells, contributing to advancements in protein engineering, functional studies, and the development of therapeutic agents.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

His-Tag Antibody (His17)

sc-51946
100 µg/ml
$316.00

What is the total volume provided of His-probe (His17): sc-51946 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2018-05-23
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Rated 5 out of 5 by from nice probe antibodyuse it in WB,the data is great.but this antibody still have some background after long time exposure.so the sugesstion is no long time expure.dulution 1:1000
Date published: 2016-11-13
Rated 4 out of 5 by from Publishable data obtained by Western BlotPublishable data obtained by Western Blot using African Green Monkey COS-7 cells. -SCBT Publication Review
Date published: 2014-07-29
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His-Tag Antibody (His17) is rated 4.5 out of 5 by 2.
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