His-Tag Antibody (HIS.H8): sc-57598

His-Tag Antibody (HIS.H8) is a mouse monoclonal IgG2b antibody raised against a 6x His-tagged polypeptide, ideal for applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). The His-tag consists of a sequence of six histidine residues added to either the N-terminus or C-terminus of a recombinant protein, and its structure is crucial for its function. The imidazole side chain of histidine has a strong affinity for metal ions like nickel and cobalt, allowing His-tagged proteins to bind efficiently to metal-chelating resins during immobilized metal affinity chromatography (IMAC). This structural property enables the purification of recombinant proteins in a single step while maintaining their native conformation and function. The small size of the His-tag minimizes interference with the protein′s structure and activity, making His-tag a preferred choice in protein expression studies. Understanding the structure and function of the His-tag is important for researchers involved in protein purification, detection, and analysis. His-Tag monoclonal antibody (HIS.H8) specifically binds to the His-tag sequence, facilitating sensitive and specific detection of His-tagged proteins in various experimental settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

His-Tag Antibody (HIS.H8) References:

1. Evaluation of chromatographic recycling for imidazole used in the chromatographic purification of His-tag recombinant proteins.  |  Noubhani, AM., et al. 2003. J Chromatogr B Analyt Technol Biomed Life Sci. 790: 153-9. PMID: 12767328
2. Pharmacokinetics of His-tag recombinant human endostatin in Rhesus monkeys.  |  Song, HF., et al. 2005. Acta Pharmacol Sin. 26: 124-8. PMID: 15659125
3. Efficient immobilization of enzymes on microchannel surface through His-tag and application for microreactor.  |  Miyazaki, M., et al. 2005. Protein Pept Lett. 12: 207-10. PMID: 15723648
4. A modified His-tag vector for the production of recombinant protein kinases.  |  Lomas-Lopez, R., et al. 2008. Anal Biochem. 377: 272-3. PMID: 18371293
5. An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules.  |  Nishimura, Y., et al. 2013. J Virol Methods. 189: 393-6. PMID: 23523889
6. Enzyme activity effects of N-terminal His-tag attached to catalytic sub-unit of phosphoinositide-3-kinase.  |  Dickson, JM., et al. 2013. Biosci Rep. 33: PMID: 23968392
7. Optimized expression in Pichia pastoris eliminates common protein contaminants from subsequent His-tag purification.  |  Chen, Y., et al. 2014. Biotechnol Lett. 36: 711-8. PMID: 24322766
8. Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coli.  |  Park, WJ., et al. 2015. Acta Biochim Biophys Sin (Shanghai). 47: 488-95. PMID: 25994007
9. A novel His-tag-binding aptamer for recombinant protein detection and T cell-based immunotherapy.  |  Yang, LT., et al. 2023. Talanta. 263: 124722. PMID: 37247456
10. N-terminal LysSN-His-tag improves the production of intracellular recombinant protein in Bacillus subtilis.  |  Le, NTP., et al. 2023. Cell Biochem Funct. 41: 823-832. PMID: 37515537

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-His-Tag Antibody (HIS.H8) has 35 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

His-Tag Antibody (HIS.H8) Specifications

Alternate Product Name: His-Tag

Product Clone Name: HIS.H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: His-probe

Epitope Species:

Product Reactivity/Detection: fusion proteins encoded by polyhistidine expression vectors

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for His-Tag Antibody (HIS.H8): N/A

The preferred His-Tag antibody is His-Tag Antibody (H-3): sc-8036.