HIF1a (m): 293T Lysate: sc-120778

The HIF-1α (m): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line known for its high transfection efficiency and versatility in research. This lysate contains Hypoxia-Inducible Factor 1-alpha (HIF-1α), a transcription factor crucial for cellular response to low oxygen levels (hypoxia). HIF-1α is regulated by oxygen availability; under normoxic conditions, it is rapidly degraded via the ubiquitin-proteasome pathway, but under hypoxic conditions, it stabilizes and translocates to the nucleus. There, it dimerizes with HIF-1β and binds to hypoxia-responsive elements in target gene promoters, activating genes involved in angiogenesis, metabolism, and cell survival. In research, the HIF-1α (m): 293T Lysate is employed to study the molecular mechanisms of hypoxia signaling and the adaptive responses to low oxygen levels. Researchers use this lysate in in vitro assays to investigate HIF-1α protein interactions, post-translational modifications such as hydroxylation and acetylation, and the transcriptional activation of hypoxia-responsive genes. This lysate is particularly valuable for exploring the regulatory pathways that control HIF-1α stability and activity, as well as the cellular processes influenced by HIF-1α signaling. Additionally, it aids in screening for potential modulators of HIF-1α activity, providing insights into the broader implications of hypoxia-induced signaling pathways in cellular physiology and adaptation.