HGK Antibody (5-12): sc-100445

HGK Antibody (5-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects HGK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HGK antibody (5-12) is available as the non-conjugated form. HGK protein, also known as HPK/GC kinase-like kinase, mitogen-activated protein kinase kinase kinase kinase 4, MAPK/ERK kinase kinase kinase 4, or NCK-interacting kinase (NIK), plays a crucial role in cellular signaling as a member of the serine/threonine kinase subfamily, Ste20. This subfamily includes several mammalian kinases that share sequence similarity with the yeast serine/threonine kinase Ste20, encompassing proteins such as KHS, GLK, YSK1, HPK1, Krs-1, Krs-2, and GC kinase. HGK activates the SAPK/JNK stress response pathway, which is vital for cellular responses to stress and inflammation. Through interaction with MEK kinase-1, HGK functions upstream in the SAPK/JNK signaling cascade, highlighting a role in regulating critical cellular processes. Both the kinase domain and C-terminal regulatory domain of HGK are essential for full activation, underscoring the intricate regulation of this signaling pathway.

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HGK Antibody (5-12) References:

1. A novel GTP-binding protein hGBP3 interacts with NIK/HGK.  |  Luan, Z., et al. 2002. FEBS Lett. 530: 233-8. PMID: 12387898
2. The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion, and adhesion.  |  Wright, JH., et al. 2003. Mol Cell Biol. 23: 2068-82. PMID: 12612079
3. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.  |  Leberer, E., et al. 1992. EMBO J. 11: 4815-24. PMID: 1464311
4. hGK-1: a kallikrein gene expressed in human prostate.  |  Morris, BJ. 1989. Clin Exp Pharmacol Physiol. 16: 345-51. PMID: 2472912
5. HGK promotes metastatic dissemination in prostate cancer.  |  Garcia-Garcia, S., et al. 2021. Sci Rep. 11: 12287. PMID: 34112843
6. Risk assessment of the inhibition of hydroxygenkwanin on human and rat cytochrome P450 by cocktail method.  |  Gao, J., et al. 2022. Toxicol In Vitro. 79: 105281. PMID: 34843882
7. Response of human gingival keratinocytes to hybrid CAD/CAM material eluates.  |  Jennes, ME., et al. 2022. Dent Mater. 38: 1532-1546. PMID: 35914965
8. Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae.  |  Wu, C., et al. 1995. J Biol Chem. 270: 15984-92. PMID: 7608157
9. Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.  |  Hu, MC., et al. 1996. Genes Dev. 10: 2251-64. PMID: 8824585
10. NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain.  |  Su, YC., et al. 1997. EMBO J. 16: 1279-90. PMID: 9135144
11. Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase.  |  Diener, K., et al. 1997. Proc Natl Acad Sci U S A. 94: 9687-92. PMID: 9275185
12. A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway.  |  Yao, Z., et al. 1999. J Biol Chem. 274: 2118-25. PMID: 9890973