Hepatocyte Specific Antigen Antibody (3D16): sc-71243

Hepatocyte Specific Antigen Antibody (3D16) is a mouse monoclonal IgG1 antibody that detects Hepatocyte Specific Antigen (HSA) in human samples through immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). HSA, also known as Hepatocyte Paraffin 1 or Hep Par 1, is primarily localized to the mitochondria of hepatocytes, playing a crucial role in the identification and diagnosis of hepatocellular carcinomas (HCC) in both humans and dogs. HSA serves as a sensitive marker that helps differentiate HCC from metastatic carcinomas and cholangiocarcinomas, with strong expression levels correlating with smaller tumor sizes and improved patient survival rates. HSA presence is particularly significant as HSA may also indicate the potential for intestinal metaplasia, a condition that can precede cancer development. While HCCs predominantly express HSA, a small percentage may not, suggesting alternative mechanisms of hepatocarcinogenesis in the early stages of the disease. HSA is found in gastric carcinomas and a few other non-hepatic tumors, underscoring HSA relevance in cancer diagnostics.

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Hepatocyte Specific Antigen Antibody (3D16) References:

1. Immunoreactivity for hepatocyte paraffin 1 antibody in hepatoid adenocarcinomas of the gastrointestinal tract.  |  Maitra, A., et al. 2001. Am J Clin Pathol. 115: 689-94. PMID: 11345832
2. Immunohistochemical characterization of canine hyperplastic hepatic lesions and hepatocellular and biliary neoplasms with monoclonal antibody hepatocyte paraffin 1 and a monoclonal antibody to cytokeratin 7.  |  Ramos-Vara, JA., et al. 2001. Vet Pathol. 38: 636-43. PMID: 11732796
3. Hepatocyte paraffin 1 expression in human normal and neoplastic tissues: tissue microarray analysis on 3,940 tissue samples.  |  Lugli, A., et al. 2004. Am J Clin Pathol. 122: 721-7. PMID: 15491968
4. Elevated hepatocyte paraffin 1 and neprilysin expression in hepatocellular carcinoma are correlated with longer survival.  |  Mondada, D., et al. 2006. Virchows Arch. 448: 35-45. PMID: 16220294
5. The utility of hepatocyte paraffin 1 antibody in the immunohistological distinction of hepatocellular carcinoma from cholangiocarcinoma and metastatic carcinoma.  |  Shiran, MS., et al. 2006. Malays J Pathol. 28: 87-92. PMID: 18376797
6. Metastatic hepatocellular carcinoma of skin diagnosed with hepatocyte paraffin 1 and a-fetoprotein immunostainings.  |  Terada, T. and Sugiura, M. 2010. Int J Surg Pathol. 18: 433-6. PMID: 18499685
7. Hepatocyte paraffin 1 antigen as a biomarker for early diagnosis of Barrett esophagus.  |  Jeung, JA., et al. 2012. Am J Clin Pathol. 137: 111-20. PMID: 22180484
8. Hepatocyte Paraffin 1 Immunoreactivity in Early Colon Carcinogenesis.  |  Nemolato, S., et al. 2009. Gastroenterology Res. 2: 277-281. PMID: 27956971
9. Glypican-3 and hepatocyte paraffin-1 combined with alpha-fetoprotein as a novel risk scoring model for predicting early recurrence of hepatocellular carcinoma after curative resection.  |  Bao, S., et al. 2021. Eur J Gastroenterol Hepatol. 33: e603-e609. PMID: 34034276
10. Arginase-1 is More Specific Than Hepatocyte Paraffin 1 for Differentiating Hepatocellular Carcinomas With Cytoplasmic Clearing from Nonhepatocellular Clear Cell Tumors in Liver Biopsies.  |  Larson, BK., et al. 2024. Appl Immunohistochem Mol Morphol. 32: 37-43. PMID: 37859468

The preferred Hepatocyte Specific Antigen antibody is Hepatocyte Specific Antigen Antibody (OCH1E5): sc-58693.