Helios Antibody (H-1) recommended for detection of Helios of mouse, rat and human origin by WB, IP, IF and ELISA

Helios Antibody (H-1): sc-390358

Helios Antibody (H-1) is rated 5.0 out of 5 by 1.
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Datasheets

    • Helios Antibody (H-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 222-280 mapping within an internal region of Helios of human origin
    • recommended for detection of Helios of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using Helios (H-1): sc-390358?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced positive Western blot data of Helios Produced positive Western blot data of Helios expression in non-transfected and human Helios transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-12-03
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