HD-PTP Antibody (F-4): sc-398711

HD-PTP Antibody (F-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects HD-PTP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HD-PTP antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HD-PTP, also known as Tyrosine-protein phosphatase non-receptor type 23 (PTP-TD14), is a 1,636 amino acid protein encoded by the human gene PTPN23 and belongs to the protein-tyrosine phosphatase family, non-receptor class subfamily. HD-PTP plays a crucial role in cellular signaling, particularly as a negative regulator of Ras-mediated mitogenic activity, which is vital for controlling cell proliferation and differentiation. The structure of HD-PTP includes one BRO1 domain, two tetratricopeptide repeat (TPR) regions, and one tyrosine-protein phosphatase domain, with the C-terminal region housing the PTP-like domain and the N-terminal region containing the TPR repeats. These structural features facilitate interactions with other proteins involved in the mitogen-activated protein kinase signaling pathway, thereby influencing cellular responses to growth factors. HD-PTP is phosphorylated in response to DNA damage, likely by ATM or ATR kinases, highlighting its role in cellular stress responses. Additionally, HD-PTP levels are modulated by angiogenic growth factors, where Vascular Endothelial Growth Factor (VEGF) does not affect expression, while Fibroblast Growth Factor-2 (FGF-2) promotes degradation through the proteasome system, underscoring the dynamic regulation of HD-PTP in various physiological contexts.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HD-PTP Antibody (F-4) References:

1. HD-PTP: A novel protein tyrosine phosphatase gene on human chromosome 3p21.3.  |  Toyooka, S., et al. 2000. Biochem Biophys Res Commun. 278: 671-8. PMID: 11095967
2. Expression analysis and modulation by HIV-Tat of the tyrosine phosphatase HD-PTP.  |  Mariotti, M., et al. 2006. J Cell Biochem. 98: 301-8. PMID: 16408268
3. The tyrosine phosphatase HD-PTP is regulated by FGF-2 through proteasome degradation.  |  Mariotti, M., et al. 2006. Front Biosci. 11: 2138-43. PMID: 16720300
4. HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions.  |  Ichioka, F., et al. 2007. Arch Biochem Biophys. 457: 142-9. PMID: 17174262
5. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.  |  Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
6. A novel protein tyrosine phosphatase expressed in lin(lo)CD34(hi)Sca(hi) hematopoietic progenitor cells.  |  Cheng, J., et al. 1996. Blood. 88: 1156-67. PMID: 8695832
7. A novel putative protein-tyrosine phosphatase contains a BRO1-like domain and suppresses Ha-ras-mediated transformation.  |  Cao, L., et al. 1998. J Biol Chem. 273: 21077-83. PMID: 9694860

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HD-PTP Antibody (F-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HD-PTP Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HD-PTP Antibody (F-4) Specifications

Alternate Product Name: HD-PTP

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 33-54

Epitope Target: HD-PTP

Epitope Species: human

Product Reactivity/Detection: HD-PTP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HD-PTP Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin