Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gα i-2 CRISPR/Cas9 KO Plasmid (h): sc-400568
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα i-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gα i-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gα i-2 Antibody (L5): sc-13534
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα i-2 CRISPR/Cas9 KO Plasmid (h) | sc-400568 | 20 µg | $397.00 |
Overview
GNAI2 encodes the human inhibitory G protein alpha subunit Gα i-2, a core transducer of GPCR signaling that suppresses adenylyl cyclase activity to reduce intracellular cAMP and modulates downstream PKA- and MAPK-linked responses. Through coupling to diverse receptors, Gα i-2 influences calcium signaling, ion channel regulation, chemotaxis, and cytoskeletal remodeling, shaping cell migration and immune-cell activation programs. Altered GNAI2 signaling has been associated with dysregulated inflammatory pathways and aberrant proliferative signaling observed across multiple disease contexts, including cancers and immune-related disorders. These properties make GNAI2 a useful node for dissecting GPCR pathway wiring, second-messenger dynamics, and context-dependent signaling plasticity in human cells.
Gα i-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GNAI2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GNAI2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GNAI2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gα i-2 protein expression.
This CRISPR knockout system enables efficient generation of GNAI2-deficient cell models for investigation of Gα i-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GNAI2 exon(s) critical for Gα i-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GNAI2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gα i-2 CRISPR/Cas9 KO Plasmid (h) and Gα i-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GNAI2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gα i-2 HDR Plasmid (h) and Gα i-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GNAI2 homology arms to support homology-directed repair at defined GNAI2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.