Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GRPR CRISPR/Cas9 KO Plasmid (h): sc-401637
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRPR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRPR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRPR Antibody (D-1): sc-398549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRPR CRISPR/Cas9 KO Plasmid (h) | sc-401637 | 20 µg | $397.00 |
Overview
GRPR (gastrin-releasing peptide receptor) is a seven-transmembrane G protein–coupled receptor that binds gastrin-releasing peptide/bombesin-like ligands to regulate intracellular calcium mobilization and downstream signaling through PLC, PKC, and MAPK cascades. GRPR activity influences cell proliferation, secretion, and neuromodulatory processes, linking it to context-dependent regulation of epithelial and neuronal physiology. Altered GRPR expression or signaling has been associated with oncogenic signaling networks and with neuroendocrine and gastrointestinal pathway dysregulation in disease-relevant models. As a membrane receptor with defined ligand responsiveness, GRPR provides a tractable node for dissecting GPCR-driven signaling, receptor trafficking, and transcriptional outputs.
GRPR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GRPR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GRPR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GRPR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRPR protein expression.
This CRISPR knockout system enables efficient generation of GRPR-deficient cell models for investigation of GRPR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GRPR exon(s) critical for GRPR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GRPR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRPR CRISPR/Cas9 KO Plasmid (h) and GRPR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GRPR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRPR HDR Plasmid (h) and GRPR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GRPR homology arms to support homology-directed repair at defined GRPR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.