group IVE sPLA2 Double Nickase Plasmid (h): sc-417296-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- group IVE sPLA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- group IVE sPLA2 Double Nickase Plasmid (h) and group IVE sPLA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLA2G4E. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
group IVE sPLA2 Double Nickase Plasmid (h) | sc-417296-NIC | 20 µg | $410.00 |
PLA2G4E encodes group IVE secretory phospholipase A2 (sPLA2), an enzyme that hydrolyzes membrane glycerophospholipids to generate free fatty acids and lysophospholipids that act as lipid second messengers. Through regulation of arachidonic acid availability, PLA2G4E influences eicosanoid biosynthesis and intersects with inflammatory signaling, membrane remodeling, and stress-responsive lipid metabolism. Altered phospholipase activity and downstream lipid mediator balance have been associated with inflammation-linked pathophysiology and tumor-associated signaling networks, making PLA2G4E a useful target for mechanistic studies in immunology and cancer biology. Its roles in shaping local lipid composition also support investigations into vesicular trafficking, barrier function, and cell-state transitions.
group IVE sPLA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLA2G4E locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLA2G4E. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLA2G4E function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLA2G4E-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.