GPR126 Double Nickase Plasmid (m): sc-432032-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR126 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR126 Double Nickase Plasmid (m) and GPR126 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Adgrg6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR126 Double Nickase Plasmid (m) | sc-432032-NIC | 20 µg | $410.00 | |||
GPR126 Double Nickase Plasmid (m2) | sc-432032-NIC-2 | 20 µg | $410.00 |
Adgrg6 encodes the adhesion G protein–coupled receptor GPR126, a membrane receptor that integrates extracellular matrix cues with intracellular G protein signaling to regulate cAMP-dependent transcriptional programs. In mouse, GPR126 is essential for Schwann cell development and peripheral nerve myelination, and it also contributes to cardiac morphogenesis and skeletal development. Through GPCR-mediated pathways and interactions with adhesion-domain ligands, GPR126 influences cell differentiation, migration, and tissue patterning. Disruption of ADGRG6/GPR126 function has been linked to developmental defects and disease-relevant phenotypes in the nervous and musculoskeletal systems, supporting mechanistic studies of myelination and morphogenesis.
GPR126 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Adgrg6 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Adgrg6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Adgrg6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Adgrg6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.