GPR105 CRISPR/Cas9 KO Plasmid (h): sc-405954
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR105 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR105 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR105 CRISPR/Cas9 KO Plasmid (h) | sc-405954 | 20 µg | $397.00 |
Overview
P2RY14 encodes GPR105, a UDP-sugar–responsive G protein-coupled receptor that senses extracellular nucleotide sugars such as UDP-glucose and related ligands. GPR105 signaling primarily couples to Gi pathways to modulate cAMP dynamics, chemotaxis, and inflammatory gene programs, linking tissue stress and metabolite release to immune cell behavior. Expression is enriched in leukocyte populations and has been associated with regulation of innate and adaptive immune responses, including macrophage and dendritic cell functions. Dysregulated P2RY14/GPR105 activity has been implicated in inflammatory microenvironments and immune-related mechanisms relevant to cardiometabolic and pulmonary disease contexts.
GPR105 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the P2RY14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P2RY14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P2RY14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR105 protein expression.
This CRISPR knockout system enables efficient generation of P2RY14-deficient cell models for investigation of GPR105 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P2RY14 exon(s) critical for GPR105 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P2RY14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR105 CRISPR/Cas9 KO Plasmid (h) and GPR105 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the P2RY14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR105 HDR Plasmid (h) and GPR105 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by P2RY14 homology arms to support homology-directed repair at defined P2RY14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.