GIT1/2 Antibody (13): sc-135925

GIT1/2 Antibody (13) is a mouse monoclonal IgG1 antibody that detects GIT1/2 protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-GIT1/2 antibody (13) is available as the non-conjugated format. GIT1 and GIT2 proteins play a crucial role in cellular signaling and vesicular trafficking, acting as GTPase-activating proteins (GAP) for the ADP ribosylation factor (ARF) family of small GTP-binding proteins. GIT1 and GIT2 proteins are primarily located in the cytoplasm and at the plasma membrane, where GIT1 and GIT2 facilitate regulation of endocytosis and exocytosis processes. Proper localization of GIT1 and GIT2 is essential for maintaining cellular homeostasis and ensuring efficient communication between cells. Dysregulation of GIT1 and GIT2 can lead to impaired receptor signaling and has been implicated in various diseases, including cancer and metabolic disorders. Using GIT1/2 (13) monoclonal antibody in research can provide valuable insights into G protein-coupled receptor signaling dynamics and vesicular transport mechanisms, ultimately contributing to a better understanding of cellular function and disease pathology.

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GIT1/2 Antibody (13) References:

1. The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing.  |  Premont, RT., et al. 2000. J Biol Chem. 275: 22373-80. PMID: 10896954
2. Characterization of the endogenous GIT1-betaPIX complex, and identification of its association to membranes.  |  Botrugno, OA., et al. 2006. Eur J Cell Biol. 85: 35-46. PMID: 16373173
3. The multifunctional GIT family of proteins.  |  Hoefen, RJ. and Berk, BC. 2006. J Cell Sci. 119: 1469-75. PMID: 16598076
4. GIT2 represses Crk- and Rac1-regulated cell spreading and Cdc42-mediated focal adhesion turnover.  |  Frank, SR., et al. 2006. EMBO J. 25: 1848-59. PMID: 16628223
5. Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues.  |  Schmalzigaug, R., et al. 2007. J Histochem Cytochem. 55: 1039-48. PMID: 17565117
6. The GIT-PIX complexes regulate the chemotactic response of rat basophilic leukaemia cells.  |  Gavina, M., et al. 2010. Biol Cell. 102: 231-44. PMID: 19912111
7. Impaired spine formation and learning in GPCR kinase 2 interacting protein-1 (GIT1) knockout mice.  |  Menon, P., et al. 2010. Brain Res. 1317: 218-26. PMID: 20043896
8. RhoJ interacts with the GIT-PIX complex and regulates focal adhesion disassembly.  |  Wilson, E., et al. 2014. J Cell Sci. 127: 3039-51. PMID: 24928894
9. Expanding functions of GIT Arf GTPase-activating proteins, PIX Rho guanine nucleotide exchange factors and GIT-PIX complexes.  |  Zhou, W., et al. 2016. J Cell Sci. 129: 1963-74. PMID: 27182061
10. GIT2-A keystone in ageing and age-related disease.  |  van Gastel, J., et al. 2018. Ageing Res Rev. 43: 46-63. PMID: 29452267

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GIT1/2 Antibody (13) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GIT1/2 Antibody (13) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GIT1/2 Antibody (13) Specifications

Alternate Product Name: GIT1

Product Clone Name: 13

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 140-472

Epitope Target: GIT2

Epitope Species: chicken

Product Reactivity/Detection: GIT1 and GIT2

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GIT1/2 Antibody (13): N/A