Gfi-1 Antibody (15-8): sc-101053
- Gfi-1 Antibody (15-8) is a mouse monoclonal IgG1 κ provided at 100 µg/ml
- raised against recombinant Gfi-1 of human origin
- recommended for detection of Gfi-1 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Gfi-1 Antibody (15-8). This work is in progress.
Gfi-1 Antibody (15-8) is a mouse monoclonal IgG1 antibody that detects Growth factor independent 1 (Gfi-1) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Gfi-1 plays a crucial role as a transcriptional repressor, specifically binding to the DNA consensus sequence TAAATCAC(A/T)GCA, which is vital for regulating gene expression involved in cell survival and differentiation. Gfi-1′s structure features six C2H2-type zinc finger motifs located at its carboxy-terminus, with zinc fingers 3, 4, and 5 being essential for DNA binding capability. A 20 amino acid SNAG domain in Gfi-1 facilitates transcriptional repression function, particularly inhibiting the pro-apoptotic gene Bax at both mRNA and protein levels, thereby preventing cell death. Beyond lymphoid system involvement, Gfi-1 is expressed in granulocytes and activated macrophages, highlighting importance in immune responses. Human Gfi-1 and related protein Gfi-1B genes, primarily expressed in bone marrow and spleen, are located on chromosomes 1p22 and 9q34.3, respectively, underscoring evolutionary conservation and functional significance in hematopoiesis and immune function.
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Gfi-1 Antibody (15-8) References:
- Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation. | Zhu, J., et al. 2002. Immunity. 16: 733-44. PMID: 12049724
- Gfi-1 oncoproteins in hematopoiesis. | Duan, Z. and Horwitz, M. 2003. Hematology. 8: 339-44. PMID: 14530176
- Gfi-1 takes center stage in hematopoietic stem cells. | Duan, Z. and Horwitz, M. 2005. Trends Mol Med. 11: 49-52. PMID: 15694865
- Identification of growth factor independent-1 (GFI1) as a repressor of 25-hydroxyvitamin D 1-alpha hydroxylase (CYP27B1) gene expression in human prostate cancer cells. | Dwivedi, PP., et al. 2005. Endocr Relat Cancer. 12: 351-65. PMID: 15947108
- Growth factor independence-1 (Gfi-1) plays a role in mediating specific granule deficiency (SGD) in a patient lacking a gene-inactivating mutation in the C/EBPepsilon gene. | Khanna-Gupta, A., et al. 2007. Blood. 109: 4181-90. PMID: 17244686
- Growth factor independent-1 maintains Notch1-dependent transcriptional programming of lymphoid precursors. | Phelan, JD., et al. 2013. PLoS Genet. 9: e1003713. PMID: 24068942
- Gfi-1 regulates the erythroid transcription factor network through Id2 repression in murine hematopoietic progenitor cells. | Kim, W., et al. 2014. Blood. 124: 1586-96. PMID: 25051963
- The role of the transcriptional repressor growth factor independent 1 in the formation of myeloid cells. | Fraszczak, J. and Möröy, T. 2017. Curr Opin Hematol. 24: 32-37. PMID: 27820734
- Growth Factor-Independent 1 Is a Tumor Suppressor Gene in Colorectal Cancer. | Chen, MS., et al. 2019. Mol Cancer Res. 17: 697-708. PMID: 30606770
- Growth Factor Independence-1 (GFI-1) Gene Expression in Hematopoietic Stem Cell Lineage Differentiation in Low Birth Weight Newborns Compared With Normal Birth Weight Newborns at Term Pregnancy. | Eram, N., et al. 2023. Cureus. 15: e50696. PMID: 38239528
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gfi-1 Antibody (15-8) | sc-101053 | 100 µg/ml | $339.00 |