Gemin3 Antibody (C-5): sc-374373

Gemin3 Antibody (C-5) is a mouse monoclonal IgG2b antibody that detects Gemin3 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Gemin3, also known as DP103, DDX20, and DEAD-box protein DP130, is a crucial component of the survival of motor neuron (SMN) complex, which is essential for the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs) in the cytoplasm and their subsequent role in pre-mRNA splicing within the nucleus. Gemin3 is located in both the cytoplasm and nucleus, with significant concentration in subnuclear structures known as gems, which are vital for the regulation of RNA processing. Gemin3′s presence in these cellular compartments underscores its importance in maintaining proper gene expression and cellular function, particularly in the context of spinal muscular atrophy, where Gemin3′s interaction with SMN is critical for motor neuron survival. Additionally, Gemin3 exhibits ATPase activity and is characterized by the conserved DEAD motif, which is indicative of its role as a putative RNA helicase. Gemin3 is expressed in various tissues, including B and T cell neuroblastoma-derived cell lines, malignant melanoma, and normal testis, while showing lower expression levels in colon, skeletal muscle, liver, kidney, and lung. Anti-Gemin3 antibody (C-5) is an invaluable tool for researchers studying the molecular mechanisms underlying neurodegenerative diseases and RNA processing.

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Gemin3 Antibody (C-5) References:

1. The Ddx20/DP103 dead box protein represses transcriptional activation by Egr2/Krox-20.  |  Gillian, AL. and Svaren, J. 2004. J Biol Chem. 279: 9056-63. PMID: 14699164
2. The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification.  |  Lee, MB., et al. 2005. Mol Cell Biol. 25: 1879-90. PMID: 15713642
3. DEAD-box protein-103 (DP103, Ddx20) is essential for early embryonic development and modulates ovarian morphology and function.  |  Mouillet, JF., et al. 2008. Endocrinology. 149: 2168-75. PMID: 18258677
4. Abnormal interaction of motor neuropathy-associated mutant HspB8 (Hsp22) forms with the RNA helicase Ddx20 (gemin3).  |  Sun, X., et al. 2010. Cell Stress Chaperones. 15: 567-82. PMID: 20157854
5. High expression of DDX20 enhances the proliferation and metastatic potential of prostate cancer cells through the NF-κB pathway.  |  Chen, W., et al. 2016. Int J Mol Med. 37: 1551-7. PMID: 27121695
6. Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer.  |  Wang, Q., et al. 2020. Comput Struct Biotechnol J. 18: 2453-2462. PMID: 33005307
7. Human bone mesenchymal stem cells-derived exosomal miRNA-361-5p alleviates osteoarthritis by downregulating DDX20 and inactivating the NF-κB signaling pathway.  |  Tao, Y., et al. 2021. Bioorg Chem. 113: 104978. PMID: 34052737
8. The combined detection of Amphiregulin, Cyclin A1 and DDX20/Gemin3 expression predicts aggressive forms of oral squamous cell carcinoma.  |  Bourova-Flin, E., et al. 2021. Br J Cancer. 125: 1122-1134. PMID: 34290392
9. Envafolimab Inhibits the Growth of Gastric Cancer Cells with Low PD-L1 Expression through the DDX20/NF-κB/TNF-α Signaling Pathway.  |  Dong, Z., et al. 2024. Curr Cancer Drug Targets.. PMID: 39021191
10. A critical genetic interaction between Gemin3/Ddx20 and translation initiation factor NAT1/eIF4G2 drives development.  |  Cacciottolo, R. and Cauchi, RJ. 2025. Dev Biol. 521: 37-51. PMID: 39924071

The preferred Gemin3 antibody is Gemin3 Antibody (12H12): sc-57007.