G_{β 1} Antibody (F-2): sc-515764

Gβ 1 Antibody (F-2) is a mouse monoclonal IgM kappa antibody that detects G beta 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gβ 1 (F-2) antibody is available as the non-conjugated form. G beta 1 protein plays a crucial role in heterotrimeric G proteins, which transmit signals from cell surface receptors to intracellular effectors. These receptors detect various extracellular stimuli, such as hormones and neurotransmitters, while effectors like adenyl cyclase generate intracellular messengers mediating cellular responses. G protein complex consists of three subunits—alpha, beta, and gamma—each encoded by distinct gene families. Among five members of the beta subunit family in mammals, G beta 1 regulates G protein alpha subunit activity and influences various signaling pathways. G beta 1 interacts with G beta 5, which, despite being only 50% homologous to other beta subunits, modulates signaling in specific tissues, including brain, pancreas, kidney, and heart. Understanding G beta 1 interactions and functions is vital for elucidating signal transduction mechanisms and their implications in various physiological processes and diseases.

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G_{β 1} Antibody (F-2) References:

1. Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.  |  Hurowitz, EH., et al. 2000. DNA Res. 7: 111-20. PMID: 10819326
2. Different beta-subunits determine G-protein interaction with transmembrane receptors.  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
3. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits.  |  Blank, JL., et al. 1992. J Biol Chem. 267: 23069-75. PMID: 1331076
4. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
5. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
6. G protein diversity is increased by associations with a variety of gamma subunits.  |  Gautam, N., et al. 1990. Proc Natl Acad Sci U S A. 87: 7973-7. PMID: 2122451
7. The heterotrimeric G protein subunits Gα(q) and Gβ(1) have lysophospholipase D activity.  |  Aoyama, C., et al. 2011. Biochem J. 440: 241-50. PMID: 21812758
8. The heterotrimeric G protein Gβ1 interacts with the catalytic subunit of protein phosphatase 1 and modulates G protein-coupled receptor signaling in platelets.  |  Pradhan, S., et al. 2017. J Biol Chem. 292: 13133-13142. PMID: 28615442
9. Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human.  |  Blatt, C., et al. 1988. Proc Natl Acad Sci U S A. 85: 7642-6. PMID: 2902634
10. Fast Inactivation of CaV2.2 Channels Is Prevented by the Gβ1 Subunit in Rat Sympathetic Neurons.  |  Reyes-Vaca, A., et al. 2017. J Mol Neurosci. 63: 377-384. PMID: 29063444
11. The Gβ1 and Gβ3 Subunits Differentially Regulate Rat Vascular Kv7 Channels.  |  Greenwood, IA. and Stott, JB. 2019. Front Physiol. 10: 1573. PMID: 31992990
12. Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels.  |  Reddy, HP., et al. 2021. iScience. 24: 103018. PMID: 34522861

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{β 1} Antibody (F-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{β 1} Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{β 1} Antibody (F-2) Specifications

Alternate Product Name: G protein subunit beta 1

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 189-201

Epitope Target: G_{β 1}

Epitope Species: human

Product Reactivity/Detection: G_{β 1}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for G_{β 1} Antibody (F-2): N/A