Date published: 2025-9-8

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GAPDH Anticorpo (G-9): sc-365062

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  • GAPDH Anticorpo G-9é um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 1.636 publicações, fornecido em 200 µg/ml
  • Produzido contra aminoácidos 1-335 que representam a completa extensão GAPDH de human origem
  • Anti-GAPDH Anticorpo (G-9) é recomendado para a detecção de GAPDH and GAPDH-2 de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-GAPDH O anticorpo anti-GAPDH (G-9) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de GAPDH (G-9): sc-365062.
  • 1 BP-HRP">m-IgG1 BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para GAPDH Antibody (G-9) para aplicações WB e IHC(P). Estes reagentes são agora oferecidos em conjuntos com GAPDH Antibody (G-9)(ver informações de encomenda abaixo).

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Anticorpo GAPDH (G-9) é um anticorpo monoclonal de camundongo IgG1 κ que detecta GAPDH e GAPDH-2 de origem de camundongo, rato e humano por WB, IP, IF, IHC(P) e ELISA. O anticorpo GAPDH (G-9) está disponível na forma de anticorpo anti-GAPDH não conjugado, bem como em múltiplas formas conjugadas de anticorpo anti-GAPDH, incluindo agarose, HRP, PE, FITC e múltiplos conjugados Alexa Fluor®. O anticorpo GAPDH (G-9) detecta especificamente a gliceraldeído-3-fosfato desidrogenase (GAPDH), também chamada de uracil DNA glicosilase. A proteína GAPDH catalisa a fosforilação oxidativa reversível do gliceraldeído-3-fosfato na presença de fosfato inorgânico e dinucleotído de adenina de nicotinamida (NAD), um passo importante para a produção de energia no metabolismo de carboidratos. Enquanto a GAPDH é reconhecida há muito tempo por desempenhar um papel integral na glicólise, funções adicionais incluem atuar como uma uracil DNA glicosilase, ativar a transcrição, ligar RNA e estar envolvida na exportação nuclear de RNA, replicação de DNA e reparo de DNA. A expressão de GAPDH é elevada em cânceres de fígado, pulmão e próstata, sugerindo que esses tecidos são controles positivos adequados para o anticorpo GAPDH (G-9). A GAPDH transloca para o núcleo durante a apoptose. Assim, a imunocoloração pelo anticorpo GAPDH (G-9) pode resultar em localização citoplasmática ou nuclear. A GAPDH forma complexos com proteínas neuronais implicadas em distúrbios neurodegenerativos humanos, incluindo o precursor β-Amiloide, Huntingtin e outras proteínas de distúrbios neuronais de repetição de trinucleotídeo.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

GAPDH Anticorpo (G-9)

sc-365062
200 µg/ml
$316.00

Pacote do GAPDH (G-9): m-IgGκ BP-HRP

sc-522129
200 µg Ab, 40 µg BP
$354.00

Pacote do GAPDH (G-9): m-IgG1 BP-HRP

sc-552050
200 µg Ab; 20 µg BP
$354.00

GAPDH Anticorpo (G-9) AC

sc-365062 AC
500 µg/ml, 25% agarose
$416.00

GAPDH Anticorpo (G-9) HRP

sc-365062 HRP
200 µg/ml
$316.00

GAPDH Anticorpo (G-9) FITC

sc-365062 FITC
200 µg/ml
$330.00

GAPDH Anticorpo (G-9) PE

sc-365062 PE
200 µg/ml
$343.00

GAPDH Anticorpo (G-9) Alexa Fluor® 488

sc-365062 AF488
200 µg/ml
$357.00

GAPDH Anticorpo (G-9) Alexa Fluor® 546

sc-365062 AF546
200 µg/ml
$357.00

GAPDH Anticorpo (G-9) Alexa Fluor® 594

sc-365062 AF594
200 µg/ml
$357.00

GAPDH Anticorpo (G-9) Alexa Fluor® 647

sc-365062 AF647
200 µg/ml
$357.00

GAPDH Anticorpo (G-9) Alexa Fluor® 680

sc-365062 AF680
200 µg/ml
$357.00

GAPDH Anticorpo (G-9) Alexa Fluor® 790

sc-365062 AF790
200 µg/ml
$357.00

We are using this antibody on mouse brain samples. We are getting a huge band at around 50kDa along with GAPDH. How can we use this antibody to get only the specific band of GAPDH?

Asked by: Shashank
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service . Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-17

这款产品二抗用哪个m-igg1还是iggk

Asked by: djxk
感谢您的提问。GAPDH (G-9) 抗体在WB 和 IHC(P) 方面的应用,推荐的二抗检测试剂为m-IgGκ BP-HRP。 Thank you for your question. m-IgGκ BP-HRP is the preferred secondary detection reagent for GAPDH Antibody (G-9) for WB and IHC(P) applications.
Answered by: Technical Service SH
Date published: 2023-10-13

Hello! I am using the antibody GAPDH (G-9) Alexa Fluor 647 (sc-365062) for Immunofluorescence. Could you please share the protocol you used for Immunofluoresnce with cultured cells (not tissue samples). Thank you!

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-10-13

why I get two clearly bands around ~55KD and ~40KD in my results,which one is specific to my antibody?thank you

Asked by: kangxin
Thank you for your question. The expected molecular weight for GAPDH is around 37-38kDa, so the band around 40kDa is your expected band.
Answered by: Tech Service
Date published: 2019-12-11

Hi, My western results detecting GAPDH around 30ish. Is it non-specific band? Thanks

Asked by: Uzma
Thank you for your question. The expected molecular weight of GapDH is 37kDa, but it can appear a little higher or lower depending on factors such as gel, markers etc. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2023-10-13

Hi, I have used SCBT Abs a lot. Can you share buffer composition that Abs are in? DTT or reducing agent is quite important for our experiment Thanks

Asked by: DrBio
Thank you for your question. GAPDH (G-9): sc-365062 contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support
Date published: 2023-10-13

How does GAPDH compare to Actin as a protein loading control for time course studies?

Asked by: Professor Griffin
Thank you for your question. Both Actin and GAPDH are generally acceptable as loading controls for time course, RNAi and covalent mod studies. However, their molecular weights are slightly different; Actin (43kda), GAPDH (37kda). In addition, GAPDH is more relevant for non-differentiated lymphocytes, whereas Actin or GAPDH may be suitable for adherents.
Answered by: Technical Support
Date published: 2024-06-18
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Rated 4 out of 5 by from GAPDH Antibody for Cytosolic FractionsThe GAPDH antibody was instrumental in defining cytosolic fractions, and the enriched, well-defined band unequivocally demonstrated its exclusive presence in this fraction. This antibody's specificity significantly contributed to the accuracy of our cytosolic protein analyses.
Date published: 2024-01-04
Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaLo utilicé en una línea celular de mosquito (C6/36) y hubo un buen reconocimiento de la proteína en WB
Date published: 2023-10-09
Rated 5 out of 5 by from GAPDH antibodyanti-glyceraldehyde 3-phosphate dehydrogenase antibody used to probe membrane and aid in monitoring protein expressions.
Date published: 2023-06-30
Rated 5 out of 5 by from a very satisfying antibodyThis antibody works well, the protein bands are very clear and easily to determine. I'm very grateful for the scientific work that has been done with this antibody.
Date published: 2023-02-07
Rated 5 out of 5 by from Worked wellWe used this antibody in brain and peripheral tissues and it worked well.
Date published: 2023-01-06
Rated 5 out of 5 by from Very good antibodyWe have been using this antibody for long time. Its very nice
Date published: 2022-06-27
Rated 5 out of 5 by from Free fatty acid receptor 2This antibody is very good.I want to use it again.
Date published: 2022-05-19
Rated 5 out of 5 by from Nice clear bandsWe use this antibody regularly in my laboratory and it gives nice clear bands, particulalry sharp when doing 12% gels. You can use this at 1:5000.
Date published: 2022-04-07
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GAPDH Anticorpo (G-9) is rated 4.7 out of 5 by 70.
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