GAPDH Antibody (FL-335) Discontinued polyclonal antibody

GAPDH Antibody (FL-335): sc-25778

GAPDH Antibody (FL-335) is rated 4.8 out of 5 by 61.
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    • GAPDH Antibody (FL-335) is a rabbit polyclonal IgG; 200 µg/ml
    • raised against amino acids 1-335 representing full length GAPDH of human origin
    • Discontinued polyclonal antibody
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GAPDH (FL-335) has been discontinued and replaced by GAPDH (0411): sc-47724.

Ordering Information

GAPDH (0411): sc-47724 [ Recommended monoclonal replacement for GAPDH (FL-335) ]
PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
GAPDH Antibody (0411) sc-47724 200 µg/ml $289.00
GAPDH Antibody (0411) Alexa Fluor® 488 sc-47724 AF488 200 µg/ml $326.00
GAPDH Antibody (0411) Alexa Fluor® 647 sc-47724 AF647 200 µg/ml $326.00
GAPDH Antibody (0411) FITC sc-47724 FITC 200 µg/ml $302.00
GAPDH Antibody (0411) HRP sc-47724 HRP 200 µg/ml $289.00
GAPDH Antibody (0411) PE sc-47724 PE 200 µg/ml $314.00

Has anyone ever produced a band at about 60 kDa in addition to the 37 kDa band? We got one for the first time recently and are wondering if anyone else has observed the same. We were using this protein as a loading control. Thank you.

Asked by: claire8886
Thank you for your question. The expected molecular weight of GAPDH is 37 kDa. We are not aware of any literature supporting a higher 60 kDa molecular weight for this protein. We would recommend including a secondary antibody control, (incubate your membrane without primary antibody), to determine if the higher molecular weight band is due to non-specific binding of your secondary antibody alone. GAPDH Antibody (FL-335): sc-25778 is a rabbit polyclonal that is now discontinued. For a limited time, we are offering a free 10 µg sample of our recommended replacement mouse monoclonal antibody, GAPDH Antibody (0411): sc-47724. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website. https://www.scbt.com/scbt/product/gapdh-antibody-0411
Answered by: Technical Support
Date published: 2017-07-26

Would it be possible to get an aliquot of this antibody? We are preparing a manuscript and do not want to change reagents that have been successful.

Asked by: MikeF
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Rated 5 out of 5 by from Great for WB Works well for WB. Used on various cell lysates including MCF7, MDA-MB-231 and 293 cells.
Date published: 2020-07-01
Rated 5 out of 5 by from GAPDH good antibody for Western blot, very clean for human cell extracts.
Date published: 2020-01-22
Rated 5 out of 5 by from Western blot This antibody worked well to detect GAPDH in our Western blot analyses
Date published: 2019-05-14
Rated 5 out of 5 by from GAPDH antibody We do not have any problems with this antibody with several cancer cell lines such as MCF7, A549 and HeLa.
Date published: 2019-03-25
Rated 5 out of 5 by from antibody The antibody worked very well in WB with U-118 MG and U-138 MG cells. Good specificity and signal strength in whole cell lysate.
Date published: 2018-05-23
Rated 4 out of 5 by from antibodies for normalization These antibodies have been to normalize the amount of protein in the different samples for western blot densitometry analyses. the work efficiently.
Date published: 2018-04-20
Rated 5 out of 5 by from Perfect antibody We've never had problems with this antibody. Works everytime, one clear band.
Date published: 2017-08-24
Rated 5 out of 5 by from Good for WB Very strong signal in Western blot. The antibody can be used much more diluted.
Date published: 2017-08-17
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