Date published: 2025-9-15
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western blotting image from recommended GAPDH (0411) monoclonal antibody replacement. Image 379463
This polyclonal antibody has been discontinued. See our recommended GAPDH (0411) monoclonal antibody (sample data shown).
western blotting image from recommended GAPDH (0411) monoclonal antibody replacement. Image 379463
This polyclonal antibody has been discontinued. See our recommended GAPDH (0411) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended GAPDH (0411) monoclonal antibody (sample data shown).

GAPDH Antibody (FL-335): sc-25778

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  • GAPDH Antibody (FL-335) is a rabbit polyclonal IgG; 200 µg/ml
  • raised against amino acids 1-335 representing full length GAPDH of human origin
  • Discontinued polyclonal antibody

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Description

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For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Ordering Information

GAPDH (0411): sc-47724 [ Recommended monoclonal replacement for GAPDH (FL-335) ]

Product NameCatalog #UNITPriceQtyFAVORITES

GAPDH Antibody (0411)

sc-47724
200 µg/ml
$316.00

GAPDH Antibody (0411): m-IgG Fc BP-HRP Bundle

sc-528511
200 µg Ab; 10 µg BP
$354.00

GAPDH Antibody (0411): m-IgGκ BP-HRP Bundle

sc-520906
200 µg Ab, 40 µg BP
$354.00

GAPDH Antibody (0411): m-IgG1 BP-HRP Bundle

sc-542968
200 µg Ab; 20 µg BP
$354.00

GAPDH Antibody (0411) AC

sc-47724 AC
500 µg/ml, 25% agarose
$416.00

GAPDH Antibody (0411) HRP

sc-47724 HRP
200 µg/ml
$316.00

GAPDH Antibody (0411) FITC

sc-47724 FITC
200 µg/ml
$330.00

GAPDH Antibody (0411) PE

sc-47724 PE
200 µg/ml
$343.00

GAPDH Antibody (0411) Alexa Fluor® 488

sc-47724 AF488
200 µg/ml
$357.00

GAPDH Antibody (0411) Alexa Fluor® 546

sc-47724 AF546
200 µg/ml
$357.00

GAPDH Antibody (0411) Alexa Fluor® 594

sc-47724 AF594
200 µg/ml
$357.00

GAPDH Antibody (0411) Alexa Fluor® 647

sc-47724 AF647
200 µg/ml
$357.00

GAPDH Antibody (0411) Alexa Fluor® 680

sc-47724 AF680
200 µg/ml
$357.00

GAPDH Antibody (0411) Alexa Fluor® 790

sc-47724 AF790
200 µg/ml
$357.00

The GAPDH (FL-335) sc-25778 antibody is showing doublets( just below 37Kda) in my blot, alongwith few other nonspecific bands.I have tried troubleshooting multiple ways,but to no benefit.Would like to try the monoclonal antibody replacement for the same.

Asked by: NIHARIKA
Thank you for contacting Santa Cruz Biotechnology. To receive information about our sample program please email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2024-06-24

We ordered the GAPDH-FL335 antibody from Santa Cruze. Just want to know, which year we ordered it and how long we can use our antibody. Lot number -c2415 GAPDH Antibody (FL-335): sc-25778

Asked by: suman
Thank you for your question. sc-25778 GAPDH (FL-335) lot C2415 was manufactured in 2015. We have a 1 year warranty from date of purchase . If stored properly at 4 degrees our Polyclonal antibodies have been known to work for 5-10 years .
Answered by: BlakeJ
Date published: 2024-04-22

Hi to whom it may concern, i found the GAPDH rabbit antibody (FL-335) is discontinued via the website (https://www.scbt.com/p/gapdh-antibody-fl-335). I would like to ask if this discontinuity is permanent or temporary? thanks!

Asked by: HY806
All of our polyclonal antibodies have been discontinued as of 2016. For assistance with selecting one of our monoclonal alternatives, please contact our Technical Sales Department.
Answered by: Dallas Tech Service
Date published: 2021-03-18

Would it be possible to get an aliquot of this antibody? We are preparing a manuscript and do not want to change reagents that have been successful.

Asked by: MikeF
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service 1
Date published: 2021-02-02

Has anyone ever produced a band at about 60 kDa in addition to the 37 kDa band? We got one for the first time recently and are wondering if anyone else has observed the same. We were using this protein as a loading control. Thank you.

Asked by: claire8886
Thank you for your question. The expected molecular weight of GAPDH is 37 kDa. We are not aware of any literature supporting a higher 60 kDa molecular weight for this protein. We would recommend including a secondary antibody control, (incubate your membrane without primary antibody), to determine if the higher molecular weight band is due to non-specific binding of your secondary antibody alone. GAPDH Antibody (FL-335): sc-25778 is a rabbit polyclonal that is now discontinued. For a limited time, we are offering a free 10 µg sample of our recommended replacement mouse monoclonal antibody, GAPDH Antibody (0411): sc-47724. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website. https://www.scbt.com/scbt/product/gapdh-antibody-0411
Answered by: Technical Support
Date published: 2017-07-26
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Rated 5 out of 5 by from Anti-GAPDH works well for WBAnti-GAPDH works well for WB at 1.2000 in embryonic stem cells.
Date published: 2024-06-16
Rated 5 out of 5 by from GAPDHIt was a good antibody for Western blotting with little background.
Date published: 2022-09-26
Rated 5 out of 5 by from Great antibodies to WBGood antibodies to WB, at a dilution of 1: 1000, to primary mouse neurons.
Date published: 2022-07-22
Rated 5 out of 5 by from Good for WBVery good ab with virtually no background for WB detection of human GAPDH. Used as loading control.
Date published: 2022-05-20
Rated 5 out of 5 by from SC-25778This antibody performs good quality for Western analysis.
Date published: 2021-10-07
Rated 4 out of 5 by from fiche techniqueBonjour, Est ce que je peux avoir l'ancienne fiche technique de l'Anti GAPDH (Sc-25778) Rabbit?
Date published: 2021-06-08
Rated 5 out of 5 by from Great for WBWorks well for WB. Used on various cell lysates including MCF7, MDA-MB-231 and 293 cells.
Date published: 2020-07-01
Rated 5 out of 5 by from GAPDHgood antibody for Western blot, very clean for human cell extracts.
Date published: 2020-01-22
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GAPDH Antibody (FL-335) is rated 4.8 out of 5 by 67.
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