GABAA Rγ2 Double Nickase Plasmid (h): sc-402057-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rγ2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GABAA Rγ2 Double Nickase Plasmid (h) and GABAA Rγ2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GABRG2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rγ2 Double Nickase Plasmid (h) | sc-402057-NIC | 20 µg | $410.00 | |||
GABAA Rγ2 Double Nickase Plasmid (h2) | sc-402057-NIC-2 | 20 µg | $410.00 |
GABRG2 encodes the γ2 subunit of the human GABA\_A receptor, a ligand-gated chloride channel that mediates fast inhibitory synaptic transmission in the central nervous system. Incorporation of γ2 influences receptor assembly, synaptic clustering via gephyrin, and pharmacological sensitivity, shaping neuronal excitability and network oscillations. GABA\_A receptor signaling intersects with activity-dependent synaptic plasticity pathways and excitation–inhibition balance programs critical for circuit development and homeostasis. Genetic and functional perturbation of GABRG2 has been linked to neurodevelopmental and seizure-related phenotypes, supporting its relevance for mechanistic studies of inhibitory neurotransmission.
GABAA Rγ2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GABRG2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GABRG2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GABRG2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GABRG2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.