GABA T-3 CRISPR/Cas9 KO Plasmid (h): sc-403545

Overview

SLC6A11 encodes the GABA transporter 3 (GABA T-3), a Na⁺/Cl⁻-dependent solute carrier that mediates high-affinity reuptake of γ-aminobutyric acid from the extracellular space to regulate inhibitory neurotransmission. By controlling synaptic and extrasynaptic GABA availability, GABA T-3 influences neuronal excitability, tonic inhibition, and overall GABAergic circuit homeostasis. This transporter participates in neurotransmitter transport and ion-coupled membrane transport processes that intersect with glutamate–GABA metabolic balance and network-level oscillatory activity. Dysregulated GABA clearance and altered SLC6A11 expression have been studied in the context of neuropsychiatric and neurological phenotypes where inhibitory signaling balance is perturbed, supporting mechanistic investigation of GABAergic dysfunction.

GABA T-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC6A11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC6A11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC6A11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GABA T-3 protein expression.

This CRISPR knockout system enables efficient generation of SLC6A11-deficient cell models for investigation of GABA T-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting SLC6A11 exon(s) critical for GABA T-3 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple SLC6A11 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by GABA T-3 CRISPR/Cas9 KO Plasmid (h) and GABA T-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC6A11 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by GABA T-3 HDR Plasmid (h) and GABA T-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC6A11 homology arms to support homology-directed repair at defined SLC6A11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.