FUCT-VIII (m): 293T Lysate: sc-120336

The FucT-VIII (m): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line known for its high transfection efficiency and utility in molecular and cellular biology research. This lysate contains Fucosyltransferase VIII (FucT-VIII), an enzyme responsible for catalyzing the transfer of fucose, a hexose sugar, from GDP-fucose to specific glycan substrates. FucT-VIII specifically adds fucose in an α1,3 linkage to N-acetylglucosamine residues, playing a crucial role in the biosynthesis of sialyl Lewis X (sLeX) antigens. These antigens are involved in cell-cell adhesion processes mediated by selectins, which are key in leukocyte trafficking and immune response. In research, the FucT-VIII (m): 293T Lysate is utilized to study the mechanisms of glycosylation and the functional roles of fucosylated glycans. Researchers employ this lysate in in vitro assays to investigate the enzymatic activity of FucT-VIII, identify its glycan substrates, and analyze the effects of fucosylation on protein function and cell signaling. This lysate is particularly valuable in exploring the molecular pathways that regulate glycosyltransferase expression and activity. Additionally, it aids in screening for potential inhibitors or modulators of FucT-VIII, providing insights into the regulation of cell adhesion and migration. By leveraging this lysate, scientists gain a deeper understanding of the complex dynamics of glycan modification and its broader implications in cellular communication and immune response.