FOXK1 Double Nickase Plasmid (m): sc-421682-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXK1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXK1 Double Nickase Plasmid (m) and FOXK1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Foxk1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXK1 Double Nickase Plasmid (m) | sc-421682-NIC | 20 µg | $410.00 | |||
FOXK1 Double Nickase Plasmid (m2) | sc-421682-NIC-2 | 20 µg | $410.00 |
Mouse Foxk1 encodes the forkhead box transcription factor FOXK1, a chromatin-associated regulator that coordinates gene programs controlling myogenic differentiation, cell-cycle progression, and metabolic adaptation. FOXK1 integrates with transcriptional and epigenetic networks to modulate muscle lineage commitment and remodeling, influencing pathways linked to energy homeostasis and stress responses. Dysregulated FOXK1 activity has been studied in contexts of skeletal muscle development, regenerative capacity, and altered proliferative states, making it relevant for dissecting mechanisms that couple transcriptional control to tissue physiology. As a nuclear DNA-binding protein, FOXK1 provides a tractable node for mapping downstream targets and transcriptional circuitry in mouse models.
FOXK1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Foxk1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Foxk1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Foxk1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Foxk1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.