Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fatty Acid Synthase Double Nickase Plasmid (h): sc-400440-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fatty Acid Synthase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fatty Acid Synthase Double Nickase Plasmid (h) and Fatty Acid Synthase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FASN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fatty Acid Synthase Antibody (G-11): sc-48357
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fatty Acid Synthase Double Nickase Plasmid (h) | sc-400440-NIC | 20 µg | $410.00 | |||
Fatty Acid Synthase Double Nickase Plasmid (h2) | sc-400440-NIC-2 | 20 µg | $410.00 |
Human FASN encodes fatty acid synthase, a multifunctional cytosolic enzyme complex that catalyzes de novo palmitate synthesis from acetyl-CoA and malonyl-CoA using NADPH. FASN activity supports lipid homeostasis by supplying fatty acids for membrane biogenesis, protein palmitoylation, and lipid-based signaling, linking nutrient status to anabolic growth programs. It interfaces with central metabolic pathways including citrate–malate–pyruvate shuttling, NADPH production, and SREBP-driven lipogenic transcriptional networks, and is responsive to insulin and mTOR signaling. Dysregulated FASN-dependent lipogenesis is widely studied in metabolic disease, obesity-associated inflammation, hepatic steatosis, and proliferative states where altered lipid flux can reshape cellular stress responses and redox balance.
Fatty Acid Synthase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FASN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FASN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FASN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FASN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.