Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FANCI Double Nickase Plasmid (h): sc-402229-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FANCI Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FANCI Double Nickase Plasmid (h) and FANCI Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FANCI. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FANCI Antibody (A-7): sc-271316
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FANCI Double Nickase Plasmid (h) | sc-402229-NIC | 20 µg | $410.00 | |||
FANCI Double Nickase Plasmid (h2) | sc-402229-NIC-2 | 20 µg | $410.00 |
FANCI encodes a core component of the Fanconi anemia (FA) DNA repair pathway that safeguards genome stability during DNA replication. Upon replication stress or interstrand crosslink damage, FANCI forms a heterodimer with FANCD2 and is monoubiquitinated to promote recruitment of downstream repair factors involved in coordinated nucleolytic processing, translesion synthesis, and homologous recombination. Through these functions, FANCI contributes to stalled replication fork protection, S-phase checkpoint signaling, and suppression of chromosomal aberrations. Disruption of FA pathway genes, including FANCI, is linked to Fanconi anemia and broader cancer-relevant DNA repair defects, making FANCI a useful node for studying genome instability mechanisms.
FANCI Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FANCI locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FANCI. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FANCI function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FANCI-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.