FANCG siRNA and shRNA Plasmids (m)

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Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Mouse	Fancg	60534	4 A5	XM_006538127, XM_006538128, XM_006538132, XM_017320336, NM_001163233, NM_053081	Q9EQR6	N/A

Description

10 µM, 50-100 transfections
siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
for independent verification of FANCG gene silencing results, individual siRNA duplex components are also available upon request
suitable control antibody: FANCG Antibody (F-8): sc-393382
sqRT-PCR Primer also available: FANCG (m)-PR: sc-145065-PR, 20 µl
Get details about si/shRNA Gene Silencers

See Protocol Online.

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Description

20 µl of each primer set is provided at a concentration of 10 µM
FANCG Primer (m) includes A set (forward & reverse) and B set (forward & reverse) primers
Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Description

20 µg, up to 20 transfections
shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to FANCG siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
shRNA Plasmids are intended only for non-commercial use.
suitable control antibody: FANCG Antibody (F-8): sc-393382
sqRT-PCR Primer also available: FANCG (m)-PR: sc-145065-PR, 20 µl

See Protocol Online.

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Description

200 µl viral stock containing 1x 10⁶ infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to FANCG siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
Lentiviral Particles are intended for non-commercial use.

See Protocol Online.

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