FAM118A CRISPR/Cas9 KO Plasmid (h): sc-412412
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM118A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM118A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM118A CRISPR/Cas9 KO Plasmid (h) | sc-412412 | 20 µg | $397.00 |
Overview
FAM118A encodes a conserved nuclear protein implicated in fundamental RNA metabolism, including RNA processing and ribonucleoprotein complex function, consistent with a role in maintaining gene expression homeostasis. Emerging functional studies suggest FAM118A contributes to regulation of cell growth and survival programs, linking it to pathways that support proliferative capacity and stress adaptation. Altered expression of FAM118A has been reported in multiple disease contexts, particularly in cancers, where it may associate with transcriptional and post-transcriptional remodeling. As a result, FAM118A is of interest for dissecting how RNA regulatory networks influence cellular phenotypes relevant to transformation and tissue homeostasis.
FAM118A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM118A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM118A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM118A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM118A protein expression.
This CRISPR knockout system enables efficient generation of FAM118A-deficient cell models for investigation of FAM118A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM118A exon(s) critical for FAM118A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM118A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM118A CRISPR/Cas9 KO Plasmid (h) and FAM118A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM118A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM118A HDR Plasmid (h) and FAM118A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM118A homology arms to support homology-directed repair at defined FAM118A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.