Ethidium Bromide Solution CAS: 1239-45-8
MF: C21H20N3•Br
MW: 394.31

Ethidium Bromide Solution (CAS 1239-45-8)

Ethidium Bromide Solution | CAS 1239-45-8 is rated 5.0 out of 5 by 1.
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Alternate Names: Ethidium Bromide Solution is also known as ETBR.
Application: Ethidium Bromide Solution is a nucleic acid stain for PAGE or agarose gel electrophoresis, and an inhibitor of DNA polymerase.
CAS Number: 1239-45-8
Molecular Weight: 394.31
Molecular Formula: C21H20N3•Br
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ethidium bromide is commonly used as a nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of Ethidium Bromide increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Because of the binding to DNA, Ethidium Bromide is a powerful inhibitor of DNA polymerase. Ethidium Bromide has also been used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.


References

1. Elliott, W.H. 1963. Biochem. J. 86: 562-567. PMID: 16748992
2. LePecq, J.B. and Paoletti, C. 1967. J. Mol. Biol. 27: 87-106. PMID: 6033613
3. Douthart, R.J., et al. 1973. Biochemistry. 12: 214-220. PMID: 4630220
4. El Hamalawi, A.R., et al. 1975. Anal. Biochem. 67: 384-391. PMID: 1163763
5. Karsten, U. and Wollenberger, A. 1977. Anal. Biochem. 77: 464-470. PMID: 842829
6. Laine, B., et al. 1984. Biochem. Biophys. Res. Commun. 119: 1147-1153. PMID: 6370250
7. Moore, S.P. and Sutherland, B.M. 1985. Anal. Biochem. 144: 15-19. PMID: 2858989
8. Scaria, P.V. and Shafer, R.H. 1991. J. Biol. Chem. 266: 5417-5423. PMID: 2005088

Formulation :
10 mg/ml in water
Physical State :
Liquid
Solubility :
Soluble in water, and 750 parts chloroform at 20° C.
Storage :
Store at 4° C
Melting Point :
0° C
Boiling Point :
100° C
IC50 :
Hepatitis C virus: IC50 = 810 nM; A2780 : IC50 = 100 nM (human); SK-OV-3: IC50 = 400 nM (human); CH1: IC50 = 400 nM (human); Telomerase reverse transcriptase: IC50 = 2500 nM (human); Hepatitis C virus: EC5050 = 600 nM
Ki Data :
DNA: Ki= 360000 M-1
Fluoresence :
λex 480 nm, λem 620 nm in water
pK Values :
pKb: 1.67 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
RTECS :
SF7950000
PubChem CID :
14710
Merck Index :
14: 4731
MDL Number :
MFCD00011724
EC Number :
214-984-6
Beilstein Registry :
3642536
SMILES :
CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]

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Certificate of Analysis

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Ethidium Bromide Solution (CAS 1239-45-8) Product Citations

See how others have used Ethidium Bromide Solution (CAS 1239-45-8). Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 28927098  Zhuang, Z. et al. 2017. Oncol Lett. 14: 3437-3444.

PMID: # 26276565  Pandurangan, M. et al. 2016. Biol Trace Elem Res. 170: 309-19.

PMID: # 26985734  Pandurangan, M. et al. 2016. J. Photochem. Photobiol. B, Biol. 158: 206-11.

Citations 1 to 3 of 3 total
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Rated 5 out of 5 by from Various publications cite the use of ethidium Various publications cite the use of ethidium boromide as a nucleic acid stain for gel electrophoresis. -SCBT Publication Review
Date published: 2015-04-06
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