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western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721 — Este anticuerpo policlonal ha dejado de fabricarse. Consulta nuestro anticuerpo monoclonal recomendado Estrogen Receptor alpha (F-10) (se muestran datos de muestra).

Este anticuerpo policlonal ha dejado de fabricarse. Consulta nuestro anticuerpo monoclonal recomendado Estrogen Receptor alpha (F-10) (se muestran datos de muestra).

ERα Anticuerpo (MC-20): sc-542

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ER alpha Anticuerpo MC-20es un policlonal de conejo IgG proporcionado como 100 µg/ml
epítopo localizado en el C-terminus de ERα de origen mouse
Anticuerpo policlonal discontinuado

Ver las publicaciones del producto (298)

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Más Estrogen Receptor Anticuerpos incluyendo Estrogen Receptor alpha, ER β, ERR α, ERR β y ERR γ

Información sobre pedidos

Estrogen Receptor alpha (F-10): sc-8002 [ Monoclonal recomendado como alternativa a ERα (MC-20) ]

Nombre del producto	Número de catálogo	UNIDAD	Precio	CANTIDAD	Favoritos
Estrogen Receptor alpha Anticuerpo (F-10)	sc-8002	200 µg/ml	$316.00
Paquete de Estrogen Receptor alpha (F-10): m-IgG Fc BP-HRP	sc-528195	200 µg Ab; 10 µg BP	$354.00
Paquete de Estrogen Receptor alpha (F-10): m-IgG_2a BP-HRP	sc-547044	200 µg Ab; 10 µg BP	$354.00
Paquete de Estrogen Receptor alpha (F-10): m-IgGκ BP-HRP	sc-520517	200 µg Ab, 40 µg BP	$354.00
Estrogen Receptor alpha Anticuerpo (F-10) X	sc-8002 X	200 µg/0.1 ml	$316.00
Estrogen Receptor alpha Anticuerpo (F-10) AC	sc-8002 AC	500 µg/ml, 25% agarose	$416.00
Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 546	sc-8002 AF546	200 µg/ml	$357.00
Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 594	sc-8002 AF594	200 µg/ml	$357.00
Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 680	sc-8002 AF680	200 µg/ml	$357.00
Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 790	sc-8002 AF790	200 µg/ml	$357.00
Estrogen Receptor alpha (F-10) péptido neutralizante	sc-8002 P	100 µg/0.5 ml	$68.00

sc-8002 is not in the mouse tissues, I tried so hard with different antigen retrieval conditions, for both paraffin and cryosections on the mouse mammary gland. Really headache that SC542 is not available anymore.

Preguntado por: slimpopo

Thank you for your question. The ERα Antibody (F-10): sc-8002 is specific for an epitope mapping between amino acids 576-595 at the C-terminus of ERα of human origin and it is recommended for detection of ERα of human origin by WB, IP, IF, IHC(P) and ELISA. We do not recommend this antibody for detection of its epitope in murine samples. You can find alternative antibodies specific against the murine epitope by following this link: https://www.scbt.com/scbt/browse/er-alpha-Antibodies/_/N-1qbf0ke. For further information please contact our technical service directly.

Respondida por: SCBT Heidelberg Support

Fecha de publicación: 2019-01-24

Hi Santa Cruz Biotechnology representative, I have an ERalpha (D-4) sc-514910 that I bought in 2016. I don't see it listed on the website and I was wondering if there is a reason for this.

Preguntado por: NotTom

Thank you for your inquiry. sc-514910 is polyclonal antibody and all the polyclonal antibodies have been discontinued since 2016. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.

Respondida por: Tech Service

Fecha de publicación: 2018-08-14

Dear Santa Cruz, Why was the very good antibody against the ER-alpha receptor SC-542 discontinued ?? Look at the reference list how many studies were published with this antibody. I also worked with the antibody (cf. Kruijver et al., 2002 JCN). Sincerely

Preguntado por: Kruijver

Thank you for your question. All our polyclonal antibodies have been discontinued, we are now monoclonal specific. We offer many excellent ER-alpha specific monoclonal antibodies that are fine alternatives to sc-542, such as sc-8002, sc-787 and sc-8005. You can find them here: https://www.scbt.com/scbt/browse/estrogen-receptor-Antibodies/_/N-cuom06

Respondida por: Tech Service

Fecha de publicación: 2017-11-21

Hi, I am wondering if you guys still have the old stock of sc-542 antibody that used to be 200ug/ml? I find that that one works better for my experiments. Please let me know whether I can purchase the old stock. Thank you! Yichen

Preguntado por: Yichen

Thank you for your question. We have completely switched over all of our stock of this rabbit polyclonal, ERalpha Antibody (MC-20): sc-542 to the new concentration of 100 µg/ml. The difference in antibody performance you are experiencing may be more related to the lot number of the product, rather than the concentration. This is a rabbit polyclonal antibody and new lots are purified from different host animals. As a result, it is typical to observe variation between different lots. And each new lot must be optimized. Please note that sc-542 is going to be discontinued at the end of this year. We recommend you try one of our suggested replacement mouse monoclonal antibodies against ERalpha. Please contact our Technical Service Department and we can send you a free sample to test of the best antibody for your research needs. Please call us at (800) 457-3801 option 2, email scbt@scbt.com, or connect with us on live chat.

Respondida por: Technical Support

Fecha de publicación: 2016-10-05

Rated 5 de 5 por Carop de Work in IHCWorked well in Immunohistochemistry with a dillution of 1/500

Fecha de publicación: 2022-07-07

Rated 5 de 5 por Like hao de well in WBwo use it for years,and test it in some cell and tissues.for this result,wo test it in human cell

Fecha de publicación: 2016-12-05

Rated 4 de 5 por chunhui de goodworks well in frozen section of mammary gland, fixation with 4% PFA 2h

Fecha de publicación: 2016-11-30

Rated 3 de 5 por bear de not very good in ChIPit did not work well in ChIP using mouse thymocyte cells

Fecha de publicación: 2016-11-29

Rated 5 de 5 por LH_group de Great for immunohistochemistryWorked very well in immunohistochemistry (1:100) on murine mammary gland tissue (paraffin embedded with antigen retrieval)

Fecha de publicación: 2016-11-22

Rated 5 de 5 por YH de Antibody was used on mouse pancreas for immunoblotAntibody was used on mouse pancreas for immunoblot at 1:200 giving excellent result.

Fecha de publicación: 2016-02-02

Rated 5 de 5 por Hui_Ma de The antibody effect is very clearThe antibody effect is very clear, the background is low, with special perfect!

Fecha de publicación: 2015-10-14

Rated 5 de 5 por Susan de Able to publish using the antibody for WBAble to publish using the antibody for WB and IHC in mouse sample.

Fecha de publicación: 2015-09-16

ERα Anticuerpo (MC-20) Reseñas - página 2

ERα Anticuerpo (MC-20) clasificado 4.8 de 5 por 20.

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ERα Anticuerpo (MC-20): sc-542

Estrogen Receptor alpha Anticuerpo (F-10)

Paquete de Estrogen Receptor alpha (F-10): m-IgG Fc BP-HRP

Paquete de Estrogen Receptor alpha (F-10): m-IgG2a BP-HRP

Paquete de Estrogen Receptor alpha (F-10): m-IgGκ BP-HRP

Estrogen Receptor alpha Anticuerpo (F-10) X

Estrogen Receptor alpha Anticuerpo (F-10) AC

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor® 546

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor® 594

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor® 680

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor® 790

Estrogen Receptor alpha (F-10) péptido neutralizante

sc-8002 is not in the mouse tissues, I tried so hard with different antigen retrieval conditions, for both paraffin and cryosections on the mouse mammary gland. Really headache that SC542 is not available anymore.

Hi Santa Cruz Biotechnology representative, I have an ERalpha (D-4) sc-514910 that I bought in 2016. I don't see it listed on the website and I was wondering if there is a reason for this.

Dear Santa Cruz, Why was the very good antibody against the ER-alpha receptor SC-542 discontinued ?? Look at the reference list how many studies were published with this antibody. I also worked with the antibody (cf. Kruijver et al., 2002 JCN). Sincerely

Hi, I am wondering if you guys still have the old stock of sc-542 antibody that used to be 200ug/ml? I find that that one works better for my experiments. Please let me know whether I can purchase the old stock. Thank you! Yichen

Paquete de Estrogen Receptor alpha (F-10): m-IgG_2a BP-HRP

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 546

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 594

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 680

Estrogen Receptor alpha Anticuerpo (F-10) Alexa Fluor^® 790