Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EphA4 Double Nickase Plasmid (h): sc-400729-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EphA4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EphA4 Double Nickase Plasmid (h) and EphA4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPHA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EphA4 Antibody (D-4): sc-365503
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EphA4 Double Nickase Plasmid (h) | sc-400729-NIC | 20 µg | $410.00 | |||
EphA4 Double Nickase Plasmid (h2) | sc-400729-NIC-2 | 20 µg | $410.00 |
EPHA4 encodes EphA4, a receptor tyrosine kinase of the Eph/ephrin family that mediates contact-dependent signaling important for axon guidance, synaptic plasticity, and tissue boundary formation. Upon binding ephrin ligands, EphA4 triggers bidirectional signaling that modulates cytoskeletal remodeling, cell adhesion, and migration through pathways involving Rho family GTPases, MAPK cascades, and PI3K-associated signaling. In human biology, altered EphA4 signaling has been associated with dysregulated neuronal connectivity and aberrant cell motility programs relevant to neurodevelopmental and neurodegenerative contexts, as well as invasion-associated phenotypes in cancer models. These features make EPHA4 a useful node for studying receptor tyrosine kinase cross-talk, ligand-dependent signaling dynamics, and cell–cell communication mechanisms.
EphA4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPHA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPHA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPHA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPHA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.