Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ENSA Double Nickase Plasmid (h): sc-403380-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ENSA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ENSA Double Nickase Plasmid (h) and ENSA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ENSA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ENSA Antibody (L7Q): sc-81883
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENSA Double Nickase Plasmid (h) | sc-403380-NIC | 20 µg | $410.00 | |||
ENSA Double Nickase Plasmid (h2) | sc-403380-NIC-2 | 20 µg | $410.00 |
ENSA (endosulfine alpha) is a small, conserved phosphoprotein that modulates serine/threonine phosphatase activity, most notably by inhibiting PP2A-B55 complexes. Through this regulation, ENSA contributes to cell-cycle timing, mitotic entry/exit, and phosphorylation-dependent control of signaling nodes that coordinate proliferation and cellular stress responses. ENSA is embedded in kinase–phosphatase circuits linked to CDK activity and checkpoint control, making it relevant for studying mitotic fidelity and signaling robustness. Altered phosphorylation networks involving PP2A regulation are frequently implicated in cancer and neurodegenerative biology, positioning ENSA as a useful target for mechanistic pathway interrogation.
ENSA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ENSA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ENSA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ENSA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ENSA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.