EML4 Antibody (H.21): sc-130450
- EML4 Antibody (H.21) is a mouse monoclonal IgG1 κ EML4 antibody provided at 100 µg/ml
- raised against recombinant EML4 of human origin
- recommended for detection of EML4 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for EML4 Antibody (H.21). This work is in progress.
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EML4 Antibody (H.21) is a mouse monoclonal IgG1 kappa light chain antibody that detects EML4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-EML4 antibody (H.21) is available as the non-conjugated format. EML4, or Echinoderm microtubule-associated protein-like 4, is a vital cytoplasmic protein composed of 981 amino acids and characterized by the presence of nine WD repeats. EML4 plays a significant role in microtubule assembly dynamics, particularly during mitosis when expression levels increase. Through modification of assembly dynamics, EML4 contributes to microtubule elongation and flexibility, which is crucial for proper cell division and overall cellular function. EML4 is implicated in lung cancer development due to a chromosomal inversion on chromosome 2p, leading to an EML4-ALK fusion protein with anaplastic lymphoma receptor tyrosine kinase. This fusion complex is associated with oncogenic signaling pathways, highlighting EML4′s importance in both normal cellular processes and cancer pathogenesis.
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EML4 Antibody (H.21) References:
- Cloning and localization of C2orf2(ropp120), a previously unknown WD repeat protein. | Heidebrecht, HJ., et al. 2000. Genomics. 68: 348-50. PMID: 10995578
- Human EML4, a novel member of the EMAP family, is essential for microtubule formation. | Pollmann, M., et al. 2006. Exp Cell Res. 312: 3241-51. PMID: 16890222
- Echinoderm microtubule-associated protein like protein 4, a member of the echinoderm microtubule-associated protein family, stabilizes microtubules. | Houtman, SH., et al. 2007. Neuroscience. 144: 1373-82. PMID: 17196341
- Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. | Soda, M., et al. 2007. Nature. 448: 561-6. PMID: 17625570
- EML4-ALK fusion is linked to histological characteristics in a subset of lung cancers. | Inamura, K., et al. 2008. J Thorac Oncol. 3: 13-7. PMID: 18166835
- EML4-ALK fusion lung cancer: a rare acquired event. | Perner, S., et al. 2008. Neoplasia. 10: 298-302. PMID: 18320074
- EML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer. | Koivunen, JP., et al. 2008. Clin Cancer Res. 14: 4275-83. PMID: 18594010
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EML4 Antibody (H.21) | sc-130450 | 100 µg/ml | $333.00 |