ELL CRISPR/Cas9 KO Plasmid (m): sc-420159
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ELL CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ELL genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ELL Antibody (B-4): sc-398959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ELL CRISPR/Cas9 KO Plasmid (m) | sc-420159 | 20 µg | $397.00 |
Overview
Ell encodes ELL, an RNA polymerase II elongation factor that promotes processive transcription by limiting polymerase pausing and supporting productive mRNA synthesis. ELL participates in transcriptional regulatory complexes that coordinate elongation with chromatin context and RNA processing, thereby influencing gene expression programs linked to cell identity and proliferation. Disruption of ELL-dependent elongation can alter transcriptional fidelity and stress-response pathways, making it relevant for investigating mechanisms underlying dysregulated gene expression in cancer-associated transcriptional networks. In mouse models, Ell provides a tractable entry point to study elongation control across development, hematopoietic regulation, and genome-wide transcription dynamics.
ELL CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ell gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ell together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ell open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ELL protein expression.
This CRISPR knockout system enables efficient generation of Ell-deficient cell models for investigation of ELL signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ell exon(s) critical for ELL function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ell genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ELL CRISPR/Cas9 KO Plasmid (m) and ELL CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ell locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ELL HDR Plasmid (m) and ELL HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ell homology arms to support homology-directed repair at defined Ell target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.