E4BP4 Antibody (A-9): sc-74415

E4BP4 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects E4BP4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E4BP4 Antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E4BP4, also known as NFIL3, is a crucial transcriptional repressor belonging to the basic leucine zipper (bZIP) transcription factor family, which plays a significant role in regulating gene expression. E4BP4 is involved in the circadian regulation of gene transcription, as expression levels fluctuate in accordance with the circadian cycle. E4BP4 competes with PAR proteins for DNA binding in the suprachiasmatic nucleus, the brain′s circadian center, and in the liver, thereby influencing the transcription of genes that are otherwise activated by PAR transcription regulators. E4BP4′s ability to modulate gene expression in response to circadian rhythms is vital for maintaining physiological processes such as sleep-wake cycles and metabolic regulation. E4BP4 is integral to an interleukin-3-mediated signaling pathway that supports the survival of B cell progenitors, highlighting its importance in immune system function. The gene encoding human E4BP4 is located on chromosome 9q22, further emphasizing its relevance in both health and disease contexts.

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E4BP4 Antibody (A-9) References:

1. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism.  |  Mitsui, S., et al. 2001. Genes Dev. 15: 995-1006. PMID: 11316793
2. E4BP4/NFIL3, a PAR-related bZIP factor with many roles.  |  Cowell, IG. 2002. Bioessays. 24: 1023-9. PMID: 12386933
3. The transcription factor E4BP4 is not required for extramedullary pathways of NK cell development.  |  Crotta, S., et al. 2014. J Immunol. 192: 2677-88. PMID: 24534532
4. Glucocorticoid induced osteoblast apoptosis by increasing E4BP4 expression via up-regulation of Bim.  |  Chen, F., et al. 2014. Calcif Tissue Int. 94: 640-7. PMID: 24658772
5. E4BP4 inhibits AngII-induced apoptosis in H9c2 cardiomyoblasts by activating the PI3K-Akt pathway and promoting calcium uptake.  |  Chen, BC., et al. 2018. Exp Cell Res. 363: 227-234. PMID: 29331388
6. E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice.  |  Zhao, M., et al. 2018. Biochem Pharmacol. 152: 293-301. PMID: 29653076
7. E4BP4 facilitates glucocorticoid sensitivity of human bronchial epithelial cells via down-regulation of glucocorticoid receptor-beta.  |  Wang, Z., et al. 2018. Cell Immunol. 334: 31-37. PMID: 30153899
8. E4BP4-mediated inhibition of T follicular helper cell differentiation is compromised in autoimmune diseases.  |  Wang, Z., et al. 2020. J Clin Invest. 130: 3717-3733. PMID: 32191636
9. E4BP4 Regulates Hepatic Solute Carrier Family 2 Member 9 and Uric Acid Disposition in Mice.  |  Wang, Z., et al. 2022. Drug Metab Dispos. 50: 591-599. PMID: 35246462
10. The transcription factor NFIL3/E4BP4 regulates the developmental stage-specific acquisition of basophil function.  |  Park, J., et al. 2024. J Allergy Clin Immunol. 153: 132-145. PMID: 37783432

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E4BP4 Antibody (A-9) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-E4BP4 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E4BP4 Antibody (A-9) Specifications

Alternate Product Name: NFIL3

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 163-462

Epitope Target: E4BP4

Epitope Species: human

Product Reactivity/Detection: E4BP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for E4BP4 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred E4BP4 antibody is E4BP4 Antibody (C-6): sc-374451.