Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
E-cadherin Double Nickase Plasmid (m): sc-419587-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- E-cadherin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- E-cadherin Double Nickase Plasmid (m) and E-cadherin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cdh1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: E-cadherin Antibody (G-10): sc-8426
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E-cadherin Double Nickase Plasmid (m) | sc-419587-NIC | 20 µg | $410.00 | |||
E-cadherin Double Nickase Plasmid (m2) | sc-419587-NIC-2 | 20 µg | $410.00 |
Mouse Cdh1 encodes E-cadherin, a Ca2+-dependent cell–cell adhesion glycoprotein that forms adherens junctions through homophilic binding and linkage to the actin cytoskeleton via catenins. E-cadherin helps establish epithelial polarity, barrier integrity, and tissue morphogenesis, integrating with signaling networks such as Wnt/β-catenin, Hippo, and Rho GTPase pathways that coordinate junctional tension and transcriptional programs. Disruption of E-cadherin–mediated adhesion is closely associated with epithelial-to-mesenchymal transition, altered cell migration, and loss of differentiation. In mouse models, Cdh1 perturbation is widely used to study developmental defects, epithelial homeostasis, and mechanisms underlying invasive phenotypes in disease-relevant contexts.
E-cadherin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cdh1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cdh1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cdh1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cdh1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.