Date published: 2025-12-3
Visit Santa Cruz Animal Health

1-800-457-3801

SCBT Portrait Logo
Seach Input
Contact Us
Sign In Account
Quick Order
Cart (0)
E-64-d (CAS 88321-09-9) - chemical structure image
Molecular structure of E-64-d, CAS Number: 88321-09-9
E-64-d (CAS 88321-09-9) - chemical structure image
Molecular structure of E-64-d, CAS Number: 88321-09-9
Molecular structure of E-64-d, CAS Number: 88321-09-9

E-64-d (CAS 88321-09-9)

5.0(4)
Write a reviewAsk a question

See product citations (37)

Alternate Names:
E-64-d is also known as Aloxistatin.
Application:
E-64-d is a potent lysosome and cell permeable inhibitor of thiol protease and cathepsin B, H, and L.
CAS Number:
88321-09-9
Purity:
≥98%
Molecular Weight:
342.43
Molecular Formula:
C17H30N2O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

QUICK LINKS

Ordering Information
Product Citations
Description
Technical Information
Safety Information
SDS & Certificate of Analysis

E-64-d is a derivative of the membrane permeant E-64c (sc-201278) and acts as a potent inhibitor of thiol protease. It can permeate an intact cell and block the activity of calpain, which results in the protection of protein kinase C (PKC) from proteolysis. E-64-d is also a cathepsin B, cathepsin H, and cathepsin L inhibitor. Research shows that E-64-d can enhance compromised natural killer cells and bactericidal activity of leukocytes. In addition, it may act to induce cell cycle arrest during prophase and metaphase in Xenopus embryonic cells. E-64-d inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A. E-64-d is also known as EST, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester, Loxistatin, E-64d, Aloxistatin, E-64c ethyl ester, EP 453, NSC 694281, and 3-[[[(1S)-3-methyl-1[[(3S-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2S-oxiranecarboxylic acid, ethyl ester.


E-64-d (CAS 88321-09-9) References

  1. Effects of proteasome inhibitor (lactacystin) and cysteine protease inhibitor (E-64-d) on processes of mitosis in Xenopus embryonic cells.  |  Sekiguchi, T., et al. 2002. Zoolog Sci. 19: 1251-5. PMID: 12499669
  2. Amelioration of experimental arthritis by a calpain-inhibitory compound: regulation of cytokine production by E-64-d in vivo and in vitro.  |  Yoshifuji, H., et al. 2005. Int Immunol. 17: 1327-36. PMID: 16176933
  3. Influence of oestradiol and tamoxifen on oestrogen receptors-alpha and -beta protein degradation and non-genomic signalling pathways in uterine and breast carcinoma cells.  |  Horner-Glister, E., et al. 2005. J Mol Endocrinol. 35: 421-32. PMID: 16326830
  4. Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats.  |  Tsubokawa, T., et al. 2006. J Neurosci Res. 84: 832-40. PMID: 16802320
  5. Effect of a thiol proteinase inhibitor, E-64-d, on susceptibility to infection with Staphylococcus aureus in Chediak-Higashi syndrome (beige) mice.  |  Morimoto, M., et al. 2007. Int Immunopharmacol. 7: 973-80. PMID: 17499200
  6. Improvement of deficient natural killer activity and delayed bactericidal activity by a thiol proteinase inhibitor, E-64-d, in leukocytes from Chediak-Higashi syndrome patients in vitro.  |  Tanabe, F., et al. 2009. Int Immunopharmacol. 9: 366-70. PMID: 19185618
  7. Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d.  |  McGowan, EB., et al. 1989. Biochem Biophys Res Commun. 158: 432-5. PMID: 2537073
  8. Cathepsin B is a New Drug Target for Traumatic Brain Injury Therapeutics: Evidence for E64d as a Promising Lead Drug Candidate.  |  Hook, G., et al. 2015. Front Neurol. 6: 178. PMID: 26388830
  9. The protease inhibitor E64d improves ox-LDL-induced endothelial dysfunction in human aortic endothelial cells.  |  Chen, M., et al. 2018. Can J Physiol Pharmacol. 96: 120-127. PMID: 28854341
  10. Identifying purine nucleoside phosphorylase as the target of quinine using cellular thermal shift assay.  |  Dziekan, JM., et al. 2019. Sci Transl Med. 11: PMID: 30602534
  11. TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells.  |  Bestle, D., et al. 2020. Life Sci Alliance. 3: PMID: 32703818
  12. Swainsonine promotes apoptosis by impairing lysosomal function and inhibiting autophagic degradation in rat primary renal tubular epithelial cells.  |  Wang, S., et al. 2021. Chem Biol Interact. 336: 109319. PMID: 33186601
  13. Repurposing existing drugs for the treatment of COVID-19/SARS-CoV-2 infection: A review describing drug mechanisms of action.  |  Yousefi, H., et al. 2021. Biochem Pharmacol. 183: 114296. PMID: 33191206
  14. DEEMD: Drug Efficacy Estimation Against SARS-CoV-2 Based on Cell Morphology With Deep Multiple Instance Learning.  |  Saberian, MS., et al. 2022. IEEE Trans Med Imaging. 41: 3128-3145. PMID: 35622798
  15. E64d, a membrane-permeable cysteine protease inhibitor, attenuates the effects of parathyroid hormone on osteoblasts in vitro.  |  Murray, EJ., et al. 1997. Metabolism. 46: 1090-4. PMID: 9284902

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

E-64-d, 1 mg

sc-201280
1 mg
$70.00

E-64-d, 5 mg

sc-201280A
5 mg
$275.00

Hello. I am inquiring to see if the product below is available for shipping immediately upon ordering. If not, when is the expected shipping date? Thank you. E-64-d sc-201280A 5 mg

Asked by: John Ruedas
Thank you for your question. Please contact our Customer Care Department for current product availability.
Answered by: Technical Support
Date published: 2019-06-16

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. E-64-d, sc-201280, is a white solid.
Answered by: Chemical Support 4
Date published: 2017-03-14

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. E-64-d, sc-201280, sc-201280 is soluble in >10mg/ml in 100% ethanol and 20mg/ml in DMSO.
Answered by: TechService7
Date published: 2016-12-21
  • y_2025, m_11, d_13, h_10CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_201280, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 139ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Worked great with cell linesWe used this product on cell lines, with and without other treatments. It worked exactly as expected.
Date published: 2022-11-21
Rated 5 out of 5 by from Intracellular inhibition of cystein proteaseIntracellular inhibition of cystein protease with 1, 10 and 100 M - Published data
Date published: 2016-05-06
Rated 5 out of 5 by from This drug exerts its function pretty wellThis drug exerts its function pretty well in autophagy induction. Published data
Date published: 2015-11-06
Rated 5 out of 5 by from HornerHorner-Glister, E. et al. (PubMed 16326830) used E-64-d, a calpain inhibitor, to determine if calpain protease is a possible degradation pathway of ER . Since degradation was still observed, calpain protease was eliminated as the cause of ER degradation. -SCBT Publication Review
Date published: 2015-03-20
  • y_2025, m_11, d_13, h_10
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_201280, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
E-64-d is rated 5.0 out of 5 by 4.
  • y_2025, m_11, d_13, h_10
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_201280, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 125ms
  • REVIEWS, PRODUCT
1-800-457-3801
Visit Santa Cruz Animal Health|Visit San Juan Ranch
Santa Cruz Animal Health is a leading provider of animal health care products. ©2025 Santa Cruz Biotechnology Inc. All Rights Reserved.
Toll-Free: (800) 457-3801 - Terms & Conditions | Privacy Policy | Legal
“Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.