Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Desmuslin Double Nickase Plasmid (m): sc-433269-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Desmuslin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Desmuslin Double Nickase Plasmid (m) and Desmuslin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Synm. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Desmuslin Antibody (B-8): sc-376944
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Desmuslin Double Nickase Plasmid (m) | sc-433269-NIC | 20 µg | $410.00 | |||
Desmuslin Double Nickase Plasmid (m2) | sc-433269-NIC-2 | 20 µg | $410.00 |
Synm encodes desmuslin, an intermediate filament–associated protein enriched at the Z-disc that contributes to cytoskeletal integrity and mechanotransduction in striated muscle. In mouse, desmuslin interacts with components of the dystrophin–glycoprotein complex and related sarcolemmal scaffolds to coordinate myofibril organization, force transmission, and stress-responsive remodeling. These functions link Synm to pathways governing muscle cell architecture, adhesion signaling, and maintenance of contractile apparatus stability. Dysregulation of intermediate filament networks and Z-disc–associated proteins is broadly relevant to models of cardiomyopathy and skeletal muscle degeneration, making Synm a useful target for dissecting cytoskeletal failure mechanisms.
Desmuslin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Synm locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Synm. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Synm function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Synm-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.