DEP-1 Antibody (143-41): sc-21761

DEP-1 Antibody (143-41) is a mouse monoclonal IgG1 kappa light chain antibody that detects DEP-1 of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). DEP-1 (143-41) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Density-enhanced phosphatase-1 (DEP-1), also known as HPTP-eta/CD148, plays a crucial role in signal transduction within leukocytes and is integral to cellular differentiation processes. DEP-1 features an extracellular segment with eight fibronectin type III repeats, a single transmembrane segment, and a unique intracellular protein tyrosine phosphatase domain. This structural configuration allows DEP-1 to effectively interact with various signaling pathways, influencing immune cell activation and differentiation. In lymphoid organs, DEP-1 is prominently expressed on B and T cells, granulocytes, macrophages, certain dendritic cells, mature thymocytes, and neutrophils, while in non-lymphoid tissues, DEP-1 is found in fibrocytes, melanocytes, Schwann cells, and various epithelial cell types with glandular or endocrine functions. In Jurkat T cells, DEP-1 inhibits T cell receptor-mediated activation, leading to decreased expression of early activation markers such as CD69, reduced tyrosine phosphorylation of key intracellular proteins like ZAP-70, and impaired activation of mitogen-activated protein kinases. Despite intrinsic enzymatic activity, DEP-1 can paradoxically induce protein tyrosine phosphorylation in human lymphocytes and serine/threonine or tyrosine phosphorylation in certain tumor cell lines, highlighting a complex regulatory role in cellular signaling.

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DEP-1 Antibody (143-41) References:

1. Expression of the membrane protein tyrosine phosphatase CD148 in human tissues.  |  Autschbach, F., et al. 1999. Tissue Antigens. 54: 485-98. PMID: 10599888
2. Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhibits the RAS pathway by direct dephosphorylation of ERK1/2 kinases.  |  Sacco, F., et al. 2009. J Biol Chem. 284: 22048-22058. PMID: 19494114
3. The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis.  |  Spring, K., et al. 2015. Oncogene. 34: 5536-47. PMID: 25772245
4. Loss of PTPRJ/DEP-1 enhances NF2/Merlin-dependent meningioma development.  |  Waldt, N., et al. 2020. J Neurol Sci. 408: 116553. PMID: 31715329
5. Expression of DEP-1, a receptor-like protein-tyrosine-phosphatase, is enhanced with increasing cell density.  |  Ostman, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 9680-4. PMID: 7937872
6. Molecular cloning, characterization, and chromosomal localization of a novel protein-tyrosine phosphatase, HPTP eta.  |  Honda, H., et al. 1994. Blood. 84: 4186-94. PMID: 7994032
7. Cloning and characterization of rat density-enhanced phosphatase-1, a protein tyrosine phosphatase expressed by vascular cells.  |  Borges, LG., et al. 1996. Circ Res. 79: 570-80. PMID: 8781490
8. The receptor-like protein-tyrosine phosphatase DEP-1 is constitutively associated with a 64-kDa protein serine/threonine kinase.  |  Jallal, B., et al. 1997. J Biol Chem. 272: 12158-63. PMID: 9115287
9. CD148, a membrane protein tyrosine phosphatase, is able to induce tyrosine phosphorylation on human lymphocytes.  |  Palou, E., et al. 1997. Immunol Lett. 57: 101-3. PMID: 9232433
10. Functional characterization of receptor-type protein tyrosine phosphatase CD148 (HPTP eta/DEP-1) in Fc gamma receptor IIa signal transduction of human neutrophils.  |  Hundt, M. and Schmidt, RE. 1997. Eur J Immunol. 27: 3532-5. PMID: 9464844
11. CD148: a receptor-type protein tyrosine phosphatase involved in the regulation of human T cell activation.  |  Tangye, SG., et al. 1998. J Immunol. 161: 3249-55. PMID: 9759839
12. Negative regulation of human T cell activation by the receptor-type protein tyrosine phosphatase CD148.  |  Tangye, SG., et al. 1998. J Immunol. 161: 3803-7. PMID: 9780142

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DEP-1 Antibody (143-41) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-DEP-1 Antibody (143-41) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DEP-1 Antibody (143-41) Specifications

Alternate Product Name: DEP-1

Product Clone Name: 143-41

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: DEP-1

Epitope Species: human

Product Reactivity/Detection: DEP-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for DEP-1 Antibody (143-41): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin