Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDX46 CRISPR/Cas9 KO Plasmid (h): sc-407298
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX46 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDX46 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX46 Antibody (B-6): sc-514071
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX46 CRISPR/Cas9 KO Plasmid (h) | sc-407298 | 20 µg | $397.00 |
Overview
DDX46 encodes an ATP-dependent RNA helicase that functions as a core component of the U5 snRNP within the spliceosome, supporting pre-mRNA splicing and splice-site fidelity. By remodeling RNA–protein complexes during spliceosome assembly and catalytic activation, DDX46 influences transcript maturation, alternative splicing programs, and downstream proteome composition. Perturbation of spliceosomal helicase activity can shift RNA processing networks that regulate cell-cycle progression, DNA damage responses, and stress-adaptive transcriptional outputs. Dysregulated splicing machinery, including U5-associated factors, is broadly implicated in oncogenic transcriptome rewiring and neurodevelopmental and neurodegenerative disease mechanisms, making DDX46 a useful node for RNA biology studies.
DDX46 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DDX46 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDX46 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDX46 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDX46 protein expression.
This CRISPR knockout system enables efficient generation of DDX46-deficient cell models for investigation of DDX46 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDX46 exon(s) critical for DDX46 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDX46 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDX46 CRISPR/Cas9 KO Plasmid (h) and DDX46 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDX46 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDX46 HDR Plasmid (h) and DDX46 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDX46 homology arms to support homology-directed repair at defined DDX46 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.