DDO Antibody (H-6): sc-365135
- DDO Antibody (H-6) is a mouse monoclonal IgG1 κ DDO antibody provided at 200 µg/ml
- raised against amino acids 151-320 mapping near the C-terminus of DDO of human origin
- DDO Antibody (H-6) is recommended for detection of DDO of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DDO Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DDO Antibody (H-6). This work is in progress.
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SEE ALSO...
DDO Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DDO antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDO, or D-aspartate oxidase, is a 341 amino acid protein localized to peroxisomes and exists in two alternatively spliced isoforms, DDO-1 and DDO-2. DDO plays a crucial role in oxidative deamination of D-aspartate and N-methyl D-aspartate, utilizing flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide as cofactors. DDO′s enzymatic activity is significant in regulating neurotransmitter levels, which can impact various neurological functions and disorders. Human DDO shares 86% sequence similarity with its bovine counterpart, indicating a conserved function across species. DDO gene is located on human chromosome 6, a region associated with several genetic conditions, including early onset intestinal cancer and various neuropsychiatric disorders. DDO′s presence and interactions with other proteins underscore DDO′s importance in both cellular metabolism and potential implications in disease pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDO Antibody (H-6) References:
- Cellular and subcellular distribution of D-aspartate oxidase in human and rat brain. | Zaar, K., et al. 2002. J Comp Neurol. 450: 272-82. PMID: 12209855
- D-aspartate oxidase, a peroxisomal enzyme in liver of rat and man. | Van Veldhoven, PP., et al. 1991. Biochim Biophys Acta. 1073: 203-8. PMID: 1991137
- The genetic and biochemical properties of the D-amino acid oxidases in human tissues. | Barker, RF. and Hopkinson, DA. 1977. Ann Hum Genet. 41: 27-42. PMID: 21608
- Gender-related differences of mouse liver D-aspartate oxidase in the activity and response to administration of D-aspartate and peroxisome proliferators. | Nagasaki, H. 1994. Int J Biochem. 26: 415-23. PMID: 8187937
- cDNA cloning and expression of the flavoprotein D-aspartate oxidase from bovine kidney cortex. | Simonic, T., et al. 1997. Biochem J. 322 (Pt 3): 729-35. PMID: 9148742
- Structural and functional characterization of the human brain D-aspartate oxidase. | Setoyama, C. and Miura, R. 1997. J Biochem. 121: 798-803. PMID: 9163533
- C-terminal tripeptide Ser-Asn-Leu (SNL) of human D-aspartate oxidase is a functional peroxisome-targeting signal. | Amery, L., et al. 1998. Biochem J. 336 (Pt 2): 367-71. PMID: 9820813
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDO Antibody (H-6) | sc-365135 | 200 µg/ml | $316.00 | |||
DDO Antibody (H-6) AC | sc-365135 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DDO Antibody (H-6) HRP | sc-365135 HRP | 200 µg/ml | $316.00 | |||
DDO Antibody (H-6) FITC | sc-365135 FITC | 200 µg/ml | $330.00 | |||
DDO Antibody (H-6) PE | sc-365135 PE | 200 µg/ml | $343.00 | |||
DDO Antibody (H-6) Alexa Fluor® 488 | sc-365135 AF488 | 200 µg/ml | $357.00 | |||
DDO Antibody (H-6) Alexa Fluor® 546 | sc-365135 AF546 | 200 µg/ml | $357.00 | |||
DDO Antibody (H-6) Alexa Fluor® 594 | sc-365135 AF594 | 200 µg/ml | $357.00 | |||
DDO Antibody (H-6) Alexa Fluor® 647 | sc-365135 AF647 | 200 µg/ml | $357.00 | |||
DDO Antibody (H-6) Alexa Fluor® 680 | sc-365135 AF680 | 200 µg/ml | $357.00 | |||
DDO Antibody (H-6) Alexa Fluor® 790 | sc-365135 AF790 | 200 µg/ml | $357.00 |